Aerobic culture of anaerobic bacteria using antioxidants: a preliminary report

被引：45
作者：
La Scola, B. [1 ]
Khelaifia, S. [1 ]
Lagier, J. -C. [1 ]
Raoult, D. [1 ]
机构：
[1] Aix Marseille Univ, Fac Med, CNRS 7278, URMITE,UM63,IRD 198,Inserm 1095, F-13385 Marseille 05, France
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2014年 / 33卷 / 10期
关键词：
MALDI-TOF; IDENTIFICATION; GENE;
D O I：
10.1007/s10096-014-2137-4
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Antioxidants have been shown to help the growth of anaerobic bacteria. We were able to grow six anaerobe species (including Fusobacterium necrophorum and Ruminococcus gravus) and seven aerobic species all aerobically in Schaedler agar tubes and agar plates with high doses of ascorbic acid and/or glutathione. This may deeply change strategies for culturing bacteria.
引用
收藏
页码：1781 / 1783
页数：3
相关论文
共 10 条
[1]   Metagenomic Analysis of Brain Abscesses Identifies Specific Bacterial Associations [J].
Al Masalma, Mouhamad ;
Lonjon, Michel ;
Richet, Herve ;
Dufour, Henry ;
Roche, Pierre-Hugues ;
Drancourt, Michel ;
Raoult, Didier ;
Fournier, Pierre-Edouard .
CLINICAL INFECTIOUS DISEASES, 2012, 54 (02) :202-210
[2]   Improving the identification of anaerobes in the clinical microbiology laboratory through MALDI-TOF mass spectrometry [J].
Barreau, Melody ;
Pagnier, Isabelle ;
La Scola, Bernard .
ANAEROBE, 2013, 22 :123-125
[3]   Repertoire of Intensive Care Unit Pneumonia Microbiota (Publication with Expression of Concern) [J].
Bousbia, Sabri ;
Papazian, Laurent ;
Saux, Pierre ;
Forel, Jean Marie ;
Auffray, Jean-Pierre ;
Martin, Claude ;
Raoult, Didier ;
La Scola, Bernard .
PLOS ONE, 2012, 7 (02)
[4]  
Collins C, 2008, BIOMED SCI, P599
[5]   Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infections [J].
Fenollar, F ;
Roux, V ;
Stein, A ;
Drancourt, M ;
Raoult, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (03) :1018-1028
[6]  
Gorbach SL, 2005, MANDELL DOUGLAS BENN, P1854
[7]   Burden of emerging anaerobes in the MALDI-TOF and 16S.rRNA gene sequencing era [J].
La Scola, Bernard ;
Fournier, Pierre-Edouard ;
Raoult, Didier .
ANAEROBE, 2011, 17 (03) :106-112
[8]   Microbial culturomics: paradigm shift in the human gut microbiome study [J].
Lagier, J. -C. ;
Armougom, F. ;
Million, M. ;
Hugon, P. ;
Pagnier, I. ;
Robert, C. ;
Bittar, F. ;
Fournous, G. ;
Gimenez, G. ;
Maraninchi, M. ;
Trape, J. -F. ;
Koonin, E. V. ;
La Scola, B. ;
Raoult, D. .
CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (12) :1185-1193
[9]   Identification of Rare Pathogenic Bacteria in a Clinical Microbiology Laboratory: Impact of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry [J].
Seng, Piseth ;
Abat, Cedric ;
Rolain, Jean Marc ;
Colson, Philippe ;
Lagier, Jean-Christophe ;
Gouriet, Frederique ;
Fournier, Pierre Edouard ;
Drancourt, Michel ;
La Scola, Bernard ;
Raoult, Didier .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (07) :2182-2194
[10]   Axenic culture of fastidious and intracellular bacteria [J].
Singh, Sudhir ;
Eldin, Carole ;
Kowalczewska, Malgorzata ;
Raoult, Didier .
TRENDS IN MICROBIOLOGY, 2013, 21 (02) :92-99
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