The effect of intraoperative colonic lavage with NG-nitro-L-arginine methyl ester (L-NAME) on anastomotic healing in the presence of left-sided colonic obstruction in the rat

被引:16
作者
Erbil, Y [1 ]
Çalis, A [1 ]
Berber, E [1 ]
Mercan, S [1 ]
机构
[1] Univ Istanbul, Dept Gen Surg, Istanbul Fac Med, TR-34390 Istanbul, Turkey
来源
SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY | 2000年 / 30卷 / 05期
关键词
intestinal obstruction; irrigation; N-G-nitro-L-arginine methyl ester; anastomosis; wound healing; pressure; rat;
D O I
10.1007/s005950050615
中图分类号
R61 [外科手术学];
学科分类号
摘要
The effect of intraoperative colonic lavage with N-G-nitro-L-arginine methyl ester (L-NAME) on the healing of colonic anastomosis in the presence of a left-sided obstruction in the rat was investigated. Left-sided colonic obstruction was created in 144 Wistar rats. The obstruction site was excised 24h later and anastomosis was performed after either no irrigation or colonic lavage with either saline, povidone iodine (PI), short-chain fatty acids (SCFA), L-NAME, or glutamine, in 24 animals each. Animals were killed on days 3 and 6, and a 4-cm colonic segment with the anastomosis at the center was excised. Bursting pressure (BP) and hydroxyproline (HP) content were measured. In the saline, PI, and SCFA groups, BP was higher (P < 0.05, P < 0.05, and P < 0.001, respectively) and HP concentration was similar compared with controls. Both the BP and HP concentrations were higher in the glutamine group compared with controls (P < 0.001). BP was lower (P < 0.05) and HP concentration was similar in the L-NAME group compared with the control group. Colonocyte nutrition and tissue perfusion are the mainstays of anastomotic healing. Intraoperative colonic lavage with L-NAME suppresses colonic anastomotic healing in the presence of a left-sided obstruction.
引用
收藏
页码:421 / 425
页数:5
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