Isolation and characterization of 125 microsatellite DNA markers in the blacklip abalone, Haliotis rubra

被引:18
作者
Baranski, Matthew [1 ]
Rourke, Meaghan
Loughnan, Shannon
Austin, Chris
Robinson, Nick
机构
[1] Deakin Univ, Sch Ecol & Environm, Orlando, FL 32801 USA
[2] Charles Darwin Univ, Sch Sci & Primary Ind, Darwin, NT 0909, Australia
[3] Anim Genet & Genom, Dept Primary Ind, Attwood, Vic 3049, Australia
[4] AKVAFORSK, Inst Aquaculture Res AS, N-1432 As, Norway
来源
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 03期
关键词
abalone; aquaculture; genetic improvement; Haliotis rubra; library; microsatellite; null allele;
D O I
10.1111/j.1471-8286.2006.01327.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Australian abalone species are subject to wild harvest and aquaculture production. This study characterized 125 polymorphic microsatellite markers in the blacklip abalone, Haliotis rubra, and evaluated cross-species amplification in Haliotis laevigata and Haliotis coccoradiata. Segregation analysis of a mapping family revealed non-amplifying polymerase chain reaction null alleles at 34 loci. Cross-species amplification was achieved for 89 loci.
引用
收藏
页码:740 / 746
页数:7
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