Cloning of the novel gene encoding β-agarase C from a marine bacterium, Vibrio sp strain PO-303, and characterization of the gene product

被引：41

作者：

Dong, Jinhua ^{[1
]}

Hashikawa, Shinnosuke ^{[1
]}

Konishi, Takafumi ^{[1
]}

Tamaru, Yutaka ^{[1
]}

Araki, Toshiyoshi ^{[1
]}

机构：

[1] Mie Univ, Grad Sch Bioresources, Tsu, Mie 5148507, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 09期

关键词：

DEEP-SEA MICROBULBIFER; AMINO-ACID-SEQUENCES; ENZYMATIC-PROPERTIES; SP AP-2; PURIFICATION; NUCLEOTIDE; EXPRESSION; ISOLATE; AGAA;

D O I：

10.1128/AEM.00935-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The P-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. beta-Agarase C was identified as the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase family.

引用

页码：6399 / 6401

页数：3

共 23 条

[1]

AOKI T, 1990, NIPPON SUISAN GAKK, V56, P825

[2] PURIFICATION AND CHARACTERIZATION OF A NOVEL BETA-AGARASE FROM VIBRIO SP AP-2 [J].

AOKI, T ;

ARAKI, T ;

KITAMIKADO, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (02) :461-465

[3]

Araki T, 1998, J MAR BIOTECHNOL, V6, P193

[4]

Araki T, 1998, J MAR BIOTECHNOL, V6, P260

[5] COLOUR REACTIONS GIVEN BY SUGARS AND DIPHENYLAMINE-ANILINE SPRAY REAGENTS ON PAPER CHROMATOGRAMS [J].

BAILEY, RW ;

BOURNE, EJ .

JOURNAL OF CHROMATOGRAPHY, 1960, 4 (03) :206-213

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] STRUCTURE OF AGAR .1. FRACTIONATION OF A COMPLEX MIXTURE OF POLYSACCHARIDES [J].

DUCKWORT.M ;

YAPHE, W .

CARBOHYDRATE RESEARCH, 1971, 16 (01) :189-&

[8] The endo-β-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans:: two paralogue enzymes with different molecular organizations and catalytic behaviours [J].

Jam, M ;

Flament, D ;

Allouch, J ;

Potin, P ;

Thion, L ;

Kloareg, B ;

Czjzek, M ;

Helbert, W ;

Michel, G ;

Barbeyron, T .

BIOCHEMICAL JOURNAL, 2005, 385 :703-713

[9] Purification and characterization of a novel β-agarase from an alkalophilic bacterium, Alteromonas sp E-1 [J].

Kirimura, K ;

Masuda, N ;

Iwasaki, Y ;

Nakagawa, H ;

Kobayashi, R ;

Usami, S .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (04) :436-441

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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