Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy

被引：4

作者：

Qian, Yao ^{[3
]}

Wang, Jingping ^{[3
]}

Ji, Ziwan ^{[3
]}

Chen, Hao ^{[3
]}

He, Yucang ^{[3
]}

Lv, Xuling ^{[3
]}

Zhang, Zikai ^{[3
]}

Li, Tian ^{[3
]}

Pan, Tianyun ^{[4
]}

Li, Liqun ^{[3
]}

Lin, Ming ^{[1
,2
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Peoples R China

[2] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R China

[3] Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou 325000, Peoples R China

[4] Huzhou Hosp Tradit Chinese Med, Huzhou 313000, Peoples R China

来源：

BIOCELL | 2021年 / 45卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Oxidative stress; Cell activity; Autophagy-related proteins; Antioxidant enzymes; INHIBITS AUTOPHAGY; AMBRA1; ROS; PATHWAY; IMPROVEMENT; APOPTOSIS; INJURY; HEART;

D O I：

10.32604/biocell.2021.011827

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H2O2 (100 mu M, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.

引用

页码：189 / 198

页数：10

共 33 条

[31] Adipose-Derived Stem Cells Suppress Inflammation Induced by IL-1β through Down-Regulation of P2X7R Mediated by miR-373 in Chondrocytes of Osteoarthritis
Jin, Rilong
Shen, Miaoda
Yu, Liedao
Wang, Xuanwei
Lin, Xiangjin
MOLECULES AND CELLS, 2017, 40 (03) : 222 - 229
[32] Adipose-derived mesenchymal stem cells protect against CMS-induced depression-like behaviors in mice via regulating the Nrf2/HO-1 and TLR4/NF-κB signaling pathways
Huang, Xiao
Fei, Guo-qiang
Liu, Wen-juan
Ding, Jing
Wang, Yuan
Wang, Hao
Ji, Jian-lin
Wang, Xin
ACTA PHARMACOLOGICA SINICA, 2020, 41 (05) : 612 - 619
[33] Activation of Liver X Receptor Improves Viability of Adipose-Derived Mesenchymal Stem Cells to Attenuate Myocardial Ischemia Injury Through TLR4/NF-κB and Keap-1/Nrf-2 Signaling Pathways
Wang, Yabin
Li, Chunhong
Cheng, Kang
Zhang, Ran
Narsinh, Kazim
Li, Shuang
Li, Xiujuan
Qin, Xing
Zhang, Rongqing
Li, Congye
Su, Tao
Chen, Jiangwei
Cao, Feng
ANTIOXIDANTS & REDOX SIGNALING, 2014, 21 (18) : 2543 - 2557

← 1 2 3 4 →