Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy

被引：5

作者：

Qian, Yao ^{[3
]}

Wang, Jingping ^{[3
]}

Ji, Ziwan ^{[3
]}

Chen, Hao ^{[3
]}

He, Yucang ^{[3
]}

Lv, Xuling ^{[3
]}

Zhang, Zikai ^{[3
]}

Li, Tian ^{[3
]}

Pan, Tianyun ^{[4
]}

Li, Liqun ^{[3
]}

Lin, Ming ^{[1
,2
]}

机构：

[1] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Peoples R China

[2] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R China

[3] Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou 325000, Peoples R China

[4] Huzhou Hosp Tradit Chinese Med, Huzhou 313000, Peoples R China

来源：

BIOCELL | 2021年 / 45卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Oxidative stress; Cell activity; Autophagy-related proteins; Antioxidant enzymes; INHIBITS AUTOPHAGY; AMBRA1; ROS; PATHWAY; IMPROVEMENT; APOPTOSIS; INJURY; HEART;

D O I：

10.32604/biocell.2021.011827

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H2O2 (100 mu M, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.

引用

页码：189 / 198

页数：10

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