Acetyl-L-carnitine protects adipose-derived stem cells exposed to H2O2 through regulating AMBRA1-related autophagy

被引：4
|
作者：
Qian, Yao [3 ]
Wang, Jingping [3 ]
Ji, Ziwan [3 ]
Chen, Hao [3 ]
He, Yucang [3 ]
Lv, Xuling [3 ]
Zhang, Zikai [3 ]
Li, Tian [3 ]
Pan, Tianyun [4 ]
Li, Liqun [3 ]
Lin, Ming [1 ,2 ]
机构：
[1] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Peoples R China
[2] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R China
[3] Wenzhou Med Univ, Affiliated Hosp 1, Wenzhou 325000, Peoples R China
[4] Huzhou Hosp Tradit Chinese Med, Huzhou 313000, Peoples R China
来源：
BIOCELL | 2021年 / 45卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Oxidative stress; Cell activity; Autophagy-related proteins; Antioxidant enzymes; INHIBITS AUTOPHAGY; AMBRA1; ROS; PATHWAY; IMPROVEMENT; APOPTOSIS; INJURY; HEART;
D O I：
10.32604/biocell.2021.011827
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The cell activity of adipose-derived stem cells (ADSCs) is affected by the intracellular reactive oxygen species (ROS) and the level of autophagy. Previous studies reveal that acetyl-L-carnitine (ALC) possesses capacities of resisting oxidative stress and regulating autophagy. Activating molecule in Beclin1-regulated autophagy protein 1 (AMBRA1) plays a key role in initiating Beclin1-regulated autophagy. In the present study, we discovered ALC pretreatment (1 mM, 24 h) significantly increased the activity of ADSCs exposed to H2O2 (100 mu M, 2 h) in vitro with improved stemness, and reduced the production of intracellular ROS. In addition, we found for the first time that ALC treatment up-regulated autophagy of ADSCs through strengthening the expressions of Beclin1 and AMBRA1 synchronously, which might be involved in the protective effect of ALC.
引用
收藏
页码：189 / 198
页数：10
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