Three flavanols delay starch digestion by inhibiting α-amylase and binding with starch

被引：73

作者：

Jiang, Chao ^{[1
]}

Chen, Yu ^{[1
]}

Ye, Xin ^{[1
]}

Wang, Li ^{[1
]}

Shao, Jiajia ^{[1
]}

Jing, Huijuan ^{[1
]}

Jiang, Chengyu ^{[1
]}

Wang, Hongxin ^{[1
,2
]}

Ma, Chaoyang ^{[1
,2
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2021年 / 172卷

关键词：

alpha-Amylase; Inhibition mechanism; Starch; Molecular dynamics;

D O I：

10.1016/j.ijbiomac.2021.01.070

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The study aimed to reveal the different mechanisms of delaying starch digestion by ECG, EGCG and Procyanidin based on the perspective of alpha-amylase-flavanol interaction and starch-flavanol interaction. The interaction characteristics of flavanols with alpha-amylase were studied from five aspects: enzyme inhibition, kinetics, fluorescence quenching, circular dichroism (CD) and computer simulation. The IC50 of flavanols (ECG, EGCG and Procyanidin) against alpha-amylase were 172.21 +/- 0.22, 732.15 +/- 0.13 and 504.45 +/- 0.19 mu g/mL according to the results of alpha-amylase inhibition experiment, respectively. ECG and Procyanidin showed mixed inhibition against alpha-amylase, while EGCG showed non-competition against alpha-amylase. However, thermodynamic parameters, computer-based docking and dynamic simulation proved that ECG and EGCG-alpha-amylase complexs were mainly driven by van der Waals and hydrogen bonds, while Procyanidin-alpha-amylase complexs was driven by hydrophobic interaction. In addition, it was indicated, by means of starch-iodine complex spectroscopy, that flavanols inhibited the digestion of starch not only through bind with alpha-amylase but also through bind with starch. Thus, flavanols as a starch-based food additive have the potential to be employed as adjuvant therapy for diabetes. (C) 2021 Elsevier B.V. All rights reserved.

引用

页码：503 / 514

页数：12

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