To be Excluded or to Bind, that is the Question: Arginine Effects on Proteins

被引:17
|
作者
Nakakido, Makoto [1 ]
Kudou, Motonori [1 ]
Arakawa, Tsutomu [2 ]
Tsumoto, Kouhei [1 ]
机构
[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Chiba 2778562, Japan
[2] Alliance Prot Labs, Thousand Oaks, CA 91360 USA
关键词
ArgHCl; guanidine; protein aggregation; preferential interaction; solubility; surface tension; ESCHERICHIA-COLI; INCLUSION-BODIES; CRYSTAL-STRUCTURES; COLUMN CHROMATOGRAPHY; SURFACE-TENSION; HEAT-CAPACITY; AMINO-ACIDS; HYDRATION; STABILIZATION; AGGREGATION;
D O I
10.2174/138920109788488824
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In spite of its wide application to protein refolding, purification, and storage, we have not yet addressed a general solution to the mechanism of the effects of arginine hydrochloride on proteins. To elucidate the mechanism of the effects on proteins, several attempts have been reported. In this review, we would review the attempts from thermodynamic and kinetic viewpoints.
引用
收藏
页码:415 / 420
页数:6
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