Analysis of one million base pairs of Neanderthal DNA

被引：432
作者：
Green, Richard E.
Krause, Johannes
Ptak, Susan E.
Briggs, Adrian W.
Ronan, Michael T.
Simons, Jan F.
Du, Lei
Egholm, Michael
Rothberg, Jonathan M.
Paunovic, Maja
Paeaebo, Svante
机构：
[1] Max Planck Inst Evolutionary Anthropol, D-04103 Leipzig, Germany
[2] 454 Life Sci, Branford, CT 06405 USA
[3] Croatian Acad Sci & Arts, Inst Quaternary Paleontol & Geol, HR-10000 Zagreb, Croatia
来源：
NATURE | 2006年 / 444卷 / 7117期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1038/nature05336
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Neanderthals are the extinct hominid group most closely related to contemporary humans, so their genome offers a unique opportunity to identify genetic changes specific to anatomically fully modern humans. We have identified a 38,000- year- old Neanderthal fossil that is exceptionally free of contamination from modern human DNA. Direct high- throughput sequencing of a DNA extract from this fossil has thus far yielded over one million base pairs of hominoid nuclear DNA sequences. Comparison with the human and chimpanzee genomes reveals that modern human and Neanderthal DNA sequences diverged on average about 500,000 years ago. Existing technology and fossil resources are now sufficient to initiate a Neanderthal genome- sequencing effort.
引用
收藏
页码：330 / 336
页数：7
相关论文
共 50 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   A haplotype map of the human genome [J].
Altshuler, D ;
Brooks, LD ;
Chakravarti, A ;
Collins, FS ;
Daly, MJ ;
Donnelly, P ;
Gibbs, RA ;
Belmont, JW ;
Boudreau, A ;
Leal, SM ;
Hardenbol, P ;
Pasternak, S ;
Wheeler, DA ;
Willis, TD ;
Yu, FL ;
Yang, HM ;
Zeng, CQ ;
Gao, Y ;
Hu, HR ;
Hu, WT ;
Li, CH ;
Lin, W ;
Liu, SQ ;
Pan, H ;
Tang, XL ;
Wang, J ;
Wang, W ;
Yu, J ;
Zhang, B ;
Zhang, QR ;
Zhao, HB ;
Zhao, H ;
Zhou, J ;
Gabriel, SB ;
Barry, R ;
Blumenstiel, B ;
Camargo, A ;
Defelice, M ;
Faggart, M ;
Goyette, M ;
Gupta, S ;
Moore, J ;
Nguyen, H ;
Onofrio, RC ;
Parkin, M ;
Roy, J ;
Stahl, E ;
Winchester, E ;
Ziaugra, L ;
Shen, Y .
NATURE, 2005, 437 (7063) :1299-1320
[3]   Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage [J].
Béjà, O ;
Suzuki, MT ;
Koonin, EV ;
Aravind, L ;
Hadd, A ;
Nguyen, LP ;
Villacorta, R ;
Amjadi, M ;
Garrigues, C ;
Jovanovich, SB ;
Feldman, RA ;
DeLong, EF .
ENVIRONMENTAL MICROBIOLOGY, 2000, 2 (05) :516-529
[4]   GenBank [J].
Benson, Dennis A. ;
Karsch-Mizrachi, Ilene ;
Lipman, David J. ;
Ostell, James ;
Wheeler, David L. .
NUCLEIC ACIDS RESEARCH, 2006, 34 :D16-D20
[5]   The Sima de los Huesos hominids date to beyond U/Th equilibrium (>350 kyr) and perhaps to 400-500 kyr:: New radiometric dates [J].
Bischoff, JL ;
Shamp, DD ;
Aramburu, A ;
Arsuaga, JL ;
Carbonell, E ;
de Castro, JMB .
JOURNAL OF ARCHAEOLOGICAL SCIENCE, 2003, 30 (03) :275-280
[6]   A highly divergent mtDNA sequence in a Neandertal individual from Italy [J].
Caramelli, David ;
Lalueza-Fox, Caries ;
Condemi, Silvana ;
Longo, Laura ;
Milani, Lucio ;
Manfredini, Alessandro ;
Manfredini, Alessandro ;
de Saint Pierre, Michelle ;
Adoni, Francesca ;
Lari, Martina ;
Giunti, Paolo ;
Ricci, Stefano ;
Casoli, Antonella ;
Calafell, Francesc ;
Mallegni, Francesco ;
Bertranpetit, Jaume ;
Stanyon, Roscoe ;
Bertorelle, Giorgio ;
Barbujani, Guido .
CURRENT BIOLOGY, 2006, 16 (16) :R630-R632
[7]  
*CHIMP SEQ AN CONS, 2005, NATURE, V0437
[8]   Modern humans did not admix with Neanderthals during their range expansion into Europe [J].
Currat, M ;
Excoffier, L .
PLOS BIOLOGY, 2004, 2 (12) :2264-2274
[9]   Demographic history and genetic differentiation in apes [J].
Fischer, Anne ;
Pollack, Joshua ;
Thalmann, Olaf ;
Nickel, Birgit ;
Paeaebo, Svante .
CURRENT BIOLOGY, 2006, 16 (11) :1133-1138
[10]  
FRANCISCUS RG, 2002, NEANDERTHALS
12345