Rice Fertilization-Independent Endosperm1 Regulates Seed Size under Heat Stress by Controlling Early Endosperm Development

被引:165
作者
Folsom, Jing J. [1 ]
Begcy, Kevin [1 ]
Hao, Xiaojuan [2 ]
Wang, Dong [2 ]
Walia, Harkamal [1 ]
机构
[1] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA
[2] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA
基金
美国国家科学基金会;
关键词
POLYCOMB GROUP GENES; DNA METHYLATION; EXPRESSION; MAIZE; CELLULARIZATION; ARABIDOPSIS; TEMPERATURE; HOMOLOGS; GENOME; GROWTH;
D O I
10.1104/pp.113.232413
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although heat stress reduces seed size in rice (Oryza sativa), little is known about the molecular mechanisms underlying the observed reduction in seed size and yield. To elucidate the mechanistic basis of heat sensitivity and reduced seed size, we imposed a moderate (34 degrees C) and a high (42 degrees C) heat stress treatment on developing rice seeds during the postfertilization stage. Both stress treatments reduced the final seed size. At a cellular level, the moderate heat stress resulted in precocious endosperm cellularization, whereas severe heat-stressed seeds failed to cellularize. Initiation of endosperm cellularization is a critical developmental transition required for normal seed development, and it is controlled by Polycomb Repressive Complex2 (PRC2) in Arabidopsis (Arabidopsis thaliana). We observed that a member of PRC2 called Fertilization-Independent Endosperm1 (OsFIE1) was sensitive to temperature changes, and its expression was negatively correlated with the duration of the syncytial stage during heat stress. Seeds from plants overexpressing OsFIE1 had reduced seed size and exhibited precocious cellularization. The DNA methylation status and a repressive histone modification of OsFIE1 were observed to be temperature sensitive. Our data suggested that the thermal sensitivity of seed enlargement could partly be caused by altered epigenetic regulation of endosperm development during the transition from the syncytial to the cellularized state.
引用
收藏
页码:238 / 248
页数:11
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