Interferon gamma ELISA was compared with the conventional tuberculin testing for the antemortem diagnosis of bovine tuberculosis. A single intradermal tuberculin skin test (SID) was carried out as per the standard protocol on animals from which 10 ml blood was collected earlier and Mycobacterium bovis gamma interferon ELISA for cattle was performed. Out of the 100 animals tested, 36 animals (36%) were found positive by tuberculin testing and 24% (22% bovine and 2% avian tuberculin) were found positive for interferon gamma. From 36 tuberculin positive cases only 16 (44.44%) were positive for interferon gamma. Interestingly, from 64 tuberculin negative cases, 8 (12.50%) were determined to be positive for interferon gamma. It can be concluded that SID has a high rate of false-positive as well as false-negative results and the specificity of interferon gamma assay was more than SID.
机构:
Yuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Kaya, Abdullah
Icen, Hasan
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Dicle Univ, Fac Vet Med, Dept Internal Med, TR-21280 Diyarbakir, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Icen, Hasan
Tuzcu, Nevin
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Cumhuriyet Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-58140 Sivas, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Tuzcu, Nevin
Simsek, Aynur
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Dicle Univ, Fac Vet Med, Dept Internal Med, TR-21280 Diyarbakir, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Simsek, Aynur
Yesilmen, Simten
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Dicle Univ, Fac Vet Med, Dept Microbiol, TR-21280 Diyarbakir, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Yesilmen, Simten
Kochan, Akin
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Dicle Univ, Fac Vet Med, Dept Internal Med, TR-21280 Diyarbakir, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Kochan, Akin
Yildirim, Ibrahim
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Dicle Univ, Fac Vet Med, Dept Genet, TR-21280 Diyarbakir, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey
Yildirim, Ibrahim
Alan, Adil
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Prov Directorate Food Agr & Livestock, TR-21100 Diyarbakir, TurkeyYuzuncu Yil Univ, Fac Vet Med, Dept Internal Med, TR-65080 Van, Turkey