RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax

被引：46
作者：
Rocco, L. [1 ,2 ]
Valentino, I. V. [1 ]
Scapigliati, G. [3 ]
Stingo, V. [1 ,2 ]
机构：
[1] Univ Naples 2, Dept Environm Biol & Pharmaceut Sci & Technol, Caserta, Italy
[2] Univ Naples Federico II, Interuniv Ctr Reach IC REACH, Naples, Italy
[3] Univ Tuscia, Dept Environm Sci, Viterbo, Italy
来源：
CYTOTECHNOLOGY | 2014年 / 66卷 / 03期
关键词：
DLEC cell line; RAPD-PCR technique; GTS %; Genotoxicity; AMPLIFIED POLYMORPHIC DNA; ARBITRARY PRIMERS; DAMAGE; WATER; MECHANISMS; BIOMARKERS; SEEDLINGS; TOXICITY; BENZENE; MARKERS;
D O I：
10.1007/s10616-013-9586-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host-pathogen interactions. Random amplified polymorphic deoxyribonucleic acid analysis by PCR (RAPD-PCR) was used for the molecular characterization of Dicentrarchus labrax embryonic cells (DLEC) as a possible tool to detect DNA alterations in environmental genotoxic studies. We studied the DNA pattern of the DLEC fish cell line, a fibroblast-like cell line derived from European sea bass. From a total of 15 primers only six showed good discriminatory power for the amplification process on DNA samples collected from cells by three different methods (organic extraction, salting-out method and chelating agent extraction). The results obtained show that the cell line chosen for this study could be used as a possible tool for the detection of potential genotoxicity of numerous chemical compounds.
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收藏
页码：383 / 393
页数：11
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