L-lactate transport into rat heart mitochondria and reconstruction of the L-lactate/pyruvate shuttle

被引：43

作者：

Valenti, D

de Bari, L

Atlante, A

Passarella, S

机构：

[1] CNR, Ctr Studio Mitocondri & Metab Energet, I-70126 Bari, Italy

[2] Univ Bari, Dipartmento Biochim & Biol Mol, I-70126 Bari, Italy

[3] Univ Molise, Dipartimento Sci Anim Vegetali & Ambiente, I-86100 Campobasso, Italy

来源：

BIOCHEMICAL JOURNAL | 2002年 / 364卷 / 01期

关键词：

lactate mitochondrial transport; lactate mitochondrial uptake; lactate/pyruvate antiporter; reducing-equivalent transfer system;

D O I：

10.1042/bj3640101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro reconstruction of the L-lactate/pyruvate shuttle has been performed, which allows NADH oxidation outside rat heart mitochondria. Such a shuttle occurs due to the combined action of the novel mitochondrial L-lactate/pyruvate antiporter, which differs from the monocarboxylate carrier, and the mitochondrial L-lactate dehydrogenase. The rate Of L-lactate/pyruvate antiport proved to regulate the shuttle in vitro.

引用

页码：101 / 104

页数：4