Development of an efficient real-time PCR assay to quantify enterotoxin-producing staphylococci in meat products

被引：19
作者：
Rodriguez, A. [1 ]
Gordillo, R. [1 ]
Andrade, M. J. [1 ]
Cordoba, J. J. [1 ]
Rodriguez, M. [1 ]
机构：
[1] Univ Extremadura, Fac Vet Sci, Meat & Meat Prod Res Inst, Food Hyg & Safety, Caceres 10003, Spain
来源：
FOOD CONTROL | 2016年 / 60卷
关键词：
Enterotoxin-producing staphylococci; qPCR; Meat products; COAGULASE-NEGATIVE STAPHYLOCOCCI; GOATS MILK; AUREUS; FOOD; QUANTIFICATION; EXPRESSION; SAMPLES; GENES;
D O I：
10.1016/j.foodcont.2015.07.040
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A SYBR Green-based real-time PCR (qPCR) procedure for the rapid and specific detection of enterotoxin-producing Staphylococcus spp. in meat products has been developed. For this, a specific primer pair based on conserved regions of enterotoxin genes was designed for detecting most of the described staphylococcal enterotoxins. No cross-reactivity with other microorganisms or non-enterotoxin-producing Staphylococcus spp. was detected. The detection limits of the assay were about 2-40 cfu/g for artificially contaminated meat products after 8 h enrichment period at 30 degrees C. Total time for assay completion was approximately 12 h. Thus, the qPCR method offers a useful, rapid and efficient tool for screening enterotoxin-producing Staphylococci in meat products. This tool could be also used for monitoring these foodborne pathogens in food safety preventive programs. (c) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：302 / 308
页数：7
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