Scanning electron microscopy of individual nanoparticle bio-markers in liquid

被引:11
作者
Liv, Nalan [1 ]
Lazic, Ivan [1 ]
Kruit, Pieter [1 ]
Hoogenboom, Jacob P. [1 ]
机构
[1] Delft Univ Technol, Fac Sci Appl, Dept Imaging Sci & Technol, NL-2628 CJ Delft, Netherlands
关键词
Correlative light and electron microscopy; Atmospheric electron microscopy; Scanning electron microscopy; Fluorescence microscopy; Nano particle; Quantum dots; CELLS; RESOLUTION; TISSUES; LIGHT;
D O I
10.1016/j.ultramic.2013.09.002
中图分类号
TH742 [显微镜];
学科分类号
摘要
We investigated SEM imaging of nanoparticle biomarkers suspended below a thin membrane, with the ultimate goal of integrating functional fluorescence and structural SEM measurements of samples kept at ambient or hydrated conditions. In particular, we investigated how resolving power in liquid SEM is affected by the interaction of the electron beam with the membrane. Simulations with the Geant4-based Monte Carlo scheme developed by Kieft and Bosch (2008) 111 are compared to experimental results with suspended nanoparticles. For 20 nm and 50 nm thin membranes, we found a beam broadening of 1.5 nm and 3 nm, respectively, with an excellent agreement between simulations and experiments. 15 nm Au nanoparticles and bio-functionalizecl core-shell quantum dots can be individually resolved in denser clusters. We demonstrated the imaging of single EGF-conjugated quantum dots docked at filopodia during cellular uptake with both fluorescence microscopy and SEM simultaneously. These results open novel opportunities for correlating live fluorescence microscopy with structural electron microscopy. (C) 2013 Elsevier B.V. All rights reserved
引用
收藏
页码:93 / 99
页数:7
相关论文
共 26 条
  • [1] Atomic-scale electron microscopy at ambient pressure
    Creemer, J. F.
    Helveg, S.
    Hoveling, G. H.
    Ullmann, S.
    Molenbroek, A. M.
    Sarro, P. M.
    Zandbergen, H. W.
    [J]. ULTRAMICROSCOPY, 2008, 108 (09) : 993 - 998
  • [2] Electron microscopy of whole cells in liquid with nanometer resolution
    de Jonge, N.
    Peckys, D. B.
    Kremers, G. J.
    Piston, D. W.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (07) : 2159 - 2164
  • [3] de Jonge N, 2011, NAT NANOTECHNOL, V6, P695, DOI [10.1038/NNANO.2011.161, 10.1038/nnano.2011.161]
  • [4] Correlative Fluorescence Microscopy and Scanning Transmission Electron Microscopy of Quantum-Dot-Labeled Proteins in Whole Cells in Liquid
    Dukes, Madeline J.
    Peckys, Diana B.
    de Jonge, Niels
    [J]. ACS NANO, 2010, 4 (07) : 4110 - 4116
  • [5] Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots
    Giepmans, BNG
    Deerinck, TJ
    Smarr, BL
    Jones, YZ
    Ellisman, MH
    [J]. NATURE METHODS, 2005, 2 (10) : 743 - 749
  • [6] ATMOSPHERIC SCANNING ELECTRON-MICROSCOPY USING SILICON-NITRIDE THIN-FILM WINDOWS
    GREEN, ED
    KINO, GS
    [J]. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (03): : 1557 - 1558
  • [7] THEORETICAL-MODEL FOR SCANNING ELECTRON-MICROSCOPY THROUGH THIN-FILM WINDOWS
    GREEN, ED
    KINO, GS
    [J]. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (06): : 3070 - 3073
  • [8] In situ liquid-cell electron microscopy of colloid aggregation and growth dynamics
    Grogan, Joseph M.
    Rotkina, Lolita
    Bau, Haim H.
    [J]. PHYSICAL REVIEW E, 2011, 83 (06):
  • [9] Beam spreading and spatial resolution in thick organic specimens
    Hyun, Jerome K.
    Ercius, Peter
    Muller, David A.
    [J]. ULTRAMICROSCOPY, 2008, 109 (01) : 1 - 7
  • [10] Scanning electron microscope imaging in liquids - some data on electron interactions in water
    Joy, DC
    Joy, CS
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 2006, 221 (02): : 84 - 88