Effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis

Background/Aims: Probiotics and antioxidants could be alternatives to antibiotics in the prevention of bacterial infections in cirrhosis. The aim of the present study was to determine the effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora, endotoxemia, and bacterial translocation in cirrhotic rats. Methods: Twenty-nine Sprague-Dawley rats with cirrhosis induced by CCl4 and ascites received Lactobacillus johnsonii La1 10(9) cfu/day in vehicle (antioxidants: vitamin C + glutamate) (n = 10), vehicle alone (n = 11), or water (n = 8) by gavage. Another eight non-cirrhotic rats formed the control group. After 10 days of treatment, a laparotomy was performed to determine microbiological study of ileal and cecal feces, bacterial translocation, endotoxemia, and intestinal malondialdehyde (MDA) levels as index of intestinal oxidative damage. Results: Intestinal enterobacteria and enterococci, bacterial translocation (0/11 and 0/10 vs. 5/8, P < 0.01), and ileal MDA levels (P < 0.01) were lower in cirrhotic rats treated with antioxidants alone or in combination with Lactobacillus johnsonii La1 compared to cirrhotic rats receiving water. Only rats treated with antioxidants and Lactobacillus johnsonii La1 showed a decrease in endotoxemia with respect to cirrhotic rats receiving water (P < 0.05). Conclusions: Antioxidants alone or in combination with Lactobacillus johnsonii Lal can be useful in preventing bacterial translocation in cirrhosis. (C) 2002 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.