Fabrication and characterization of conductive poly (3,4-ethylenedioxythiophene) doped with hyaluronic acid/poly (L-lactic acid) composite film for biomedical application

被引：55
作者：
Wang, Shuping [1 ]
Guan, Shui [1 ]
Wang, Jing [1 ]
Liu, Hailong [2 ]
Liu, Tianqing [1 ]
Ma, Xuehu [1 ]
Cui, Zhanfeng [3 ]
机构：
[1] Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China
[2] Dalian Univ Technol, Dept Biomed Engn, Dalian 116024, Peoples R China
[3] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2017年 / 123卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Poly 3,4-ethylenedioxythiophene-hyaluronic acid/poly(L-lactic acid) composite film; Conducting polymer; PC12; cells; Neurite outgrowth; Electrical stimulation; TISSUE ENGINEERING APPLICATIONS; ELECTRICAL-STIMULATION; NEURAL TISSUE; STEM-CELLS; POLYPYRROLE/CHITOSAN POLYMERS; ELECTROCHEMICAL SYNTHESIS; NEURITE OUTGROWTH; SCHWANN-CELLS; GROWTH-FACTOR; NANOFIBERS;
D O I：
10.1016/j.jbiosc.2016.07.010
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Poly 3,4-ethylenedioxythiophene (PEDOT), a polythiophene derivative, has been proved to be modified by chemical process as biocompatible conductive polymer for biomedical applications. In this study, novel hyaluronic acid (HA) doped PEDOT nanoparticles were synthesized by the method of chemical oxidative polymerization, then conductive PEDOT-HA/poly(L-lactic acid) (PLLA) composite films were prepared. The physicochemical characteristics and biocompatibility of films were further investigated. FTIR, Raman and EDX analysis demonstrated that HA was successfully doped into PEDOT particles. Cyclic voltammograms indicated PEDOT-HA particles had favorable electrochemical stability. PEDOT-HA/PLLA films showed lower surface contact angle and faster degradation degree compared with PLLA films. Moreover, the cytotoxicity test of PEDOT-HA/PLLA films showed that neuron-like pheochromocytoma (PC12) cells adhered and spread well on the surface of PEDOT-HA/PLLA films and cell viability denoted by IVFIT assay had a significant increase. PEDOT-HA/PLLA films modified with laminin (LN) also exhibited an efficiently elongated cell morphology observed by fluorescent microscope and metallographic microscope. Furthermore, PEDOT-HA/PLLA films were subjected to different current intensity to elucidate the effect of electrical stimulation (ES) on neurite outgrowth of PC12 cells. ES (0.5 mA, 2 h) significantly promoted neurite outgrowth with an average value length of 122 +/- 5 gm and enhanced the mRNA expression of growth-associated protein (GAP43) and synaptophysin (SYP) in PC12 cells when compared with other ES groups. These results suggest that PEDOT-HA/PLLA film combined with ES are conducive to cell growth and neurite outgrowth, indicating the conductive PEDOT-HA/PLLA film may be an attractive candidate with ES for enhancing nerve regeneration in nerve tissue engineering. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
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页码：116 / 125
页数：10
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