Synthesis and characterisation of PEG modified chitosan nanocapsules loaded with thymoquinone

被引:10
作者
Kumar, Suresh Kumar Vignesh [1 ]
Devi, Ponnuswamy Renuka [1 ]
Harish, Saru [1 ]
Hemananthan, Eswaran [1 ]
机构
[1] Anna Univ Reg Campus, Dept Biotechnol, Coimbatore, Tamil Nadu, India
关键词
NANOPARTICLES; METHOTREXATE; SYSTEM;
D O I
10.1049/iet-nbt.2016.0055
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Thymoquinone (TQ), a major bioactive compound of Nigella sativa seeds has several therapeutic properties. The main drawback in bringing TQ to therapeutic application is that it has poor stability and bioavailability. Hence a suitable carrier is essential for TQ delivery. Recent studies indicate biodegradable polymers are potentially good carriers of bioactive compounds. In this study, polyethylene glycol (PEG) modified chitosan (Cs) nanocapsules were developed as a carrier for TQ. Aqueous soluble low molecular weight Cs and PEG was selected among different biodegradable polymers based on their biocompatibility and efficacy as a carrier. Optimisation of synthesis of nanocapsules was done based on particle size, PDI, encapsulation efficiency and process yield. A positive zeta potential value of +48 mV, indicating good stability was observed. Scanning electron microscope and atomic-force microscopy analysis revealed spherical shaped and smooth surfaced nanocapsules with size between 100 to 300 nm. The molecular dispersion of the TQ in Cs PEG nanocapsules was studied using X-ray powder diffraction. The Fourier transform infrared spectrum of optimised nanocapsule exhibited functional groups of both polymer and drug, confirming the presence of Cs, PEG and TQ. In vitro drug release studies showed that PEG modified Cs nanocapsules loaded with TQ had a slow and sustained release.
引用
收藏
页码:104 / 112
页数:9
相关论文
共 50 条
[41]   Pectin mediated synthesis of curcumin loaded poly(lactic acid) nanocapsules for cancer treatment [J].
Alippilakkotte, Shebi ;
Sreejith, Lisa .
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 2018, 48 :66-74
[42]   Gold nanorod-loaded (PLGA-PEG) nanocapsules as near-infrared controlled release model of anticancer therapeutics [J].
Darwish, Wael Mahmoud Ahmed ;
Bayoumi, Noha A. .
LASERS IN MEDICAL SCIENCE, 2020, 35 (08) :1729-1740
[43]   Characterisation of products from EDC-mediated PEG substitution of chitosan allows optimisation of reaction conditions [J].
Mushtaq, Asim ;
Li, Li ;
Anitha, A. ;
Grondahl, Lisbeth .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 221 :204-211
[44]   PEG modified trimethyl chitosan based nanoparticles for the codelivery of doxorubicin and iSur-pDNA [J].
Mai, Qi ;
Shen, Suqin ;
Liu, Yifu ;
Tang, Cui ;
Yin, Chunhua .
MATERIALS LETTERS, 2019, 238 :143-146
[45]   Decoupling the Effects of the Size, Wall Thickness, and Porosity of Curcumin-Loaded Chitosan Nanocapsules on Their Anticancer Efficacy: Size Is the Winner [J].
Goethals, Emma C. ;
Elbaz, Abdulkareem ;
Lopata, Andreas L. ;
Bhargava, Suresh K. ;
Bansal, Vipul .
LANGMUIR, 2013, 29 (02) :658-666
[46]   Baicalin loaded in folate-PEG modified liposomes for enhanced stability and tumor targeting [J].
Chen, Yiyin ;
Le Van Minh ;
Liu, Jianwen ;
Angelov, Borislav ;
Drechsler, Markus ;
Garamus, Vasil M. ;
Willumeit-Roemer, Regine ;
Zou, Aihua .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2016, 140 :74-82
[47]   Production and characterization of encapsulated Thymoquinone loaded bacterial cellulose/chitosan composite nano film and investigation of its in vitro antibacterial activity [J].
Bayraktar, Aytul ;
Tunc, Tuba .
JOURNAL OF FOOD AND DRUG ANALYSIS, 2024, 32 (03) :338-347
[48]   Preparation and Characterisation of Alendronate-Loaded Chitosan Microparticles Obtained Through the Spray Drying Technique [J].
Ochiuz, Lacramioara ;
Peris, Jose-Esteban .
MEDICINAL CHEMISTRY, 2009, 5 (02) :191-196
[49]   Synthesis, characterisation and intracellular imaging of PEG capped BEHP-PPV nanospheres [J].
Howes, Philip ;
Thorogate, Richard ;
Green, Mark ;
Jickells, Sue ;
Daniel, Barbara .
CHEMICAL COMMUNICATIONS, 2009, (18) :2490-2492
[50]   Collagen-chitosan scaffold modified with Au and Ag nanoparticles: Synthesis and structure [J].
Rubina, M. S. ;
Kamitov, E. E. ;
Zubavichus, Ya. V. ;
Peters, G. S. ;
Naumkin, A. V. ;
Suzer, S. ;
Vasil'kov, A. Yu. .
APPLIED SURFACE SCIENCE, 2016, 366 :365-371