SpiC is required for translocation of Salmonella pathogenicity island 2 effectors and secretion of translocon proteins SseB and SseC

被引:71
作者
Freeman, JA
Rappl, C
Kuhle, V
Hensel, M
Miller, SI
机构
[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[2] Univ Washington, Dept Med, Seattle, WA 98195 USA
[3] Univ Munich, Lehrstuhl Bakteriol, Max Von Pettenkofer Inst Hyg & Med Mikrobiol, Munich, Germany
[4] FAu Erlangen Nurnberg, Inst Klin Mikrobiol Immunol & Hyg, Erlangen, Germany
关键词
D O I
10.1128/JB.184.18.4971-4980.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Salmonella pathogenicity island 2 (SPI2) type III secretion system (TTSS) promotes Salmonella enterica serovar Typhimurium virulence for mice and increased survival and replication within eukaryotic cells. After phagocytosis, Salmonella serovar Typhimurium assembles the SPI2 TTSS to translocate over a dozen effector proteins across the phagosome membrane. SpiC has been previously shown to be a translocated effector with a large contribution to virulence (K. Uchiya, M. A. Barbieri, K. Funato, A. H. Shah, P. D. Stahl, and E. A. Groisman, EMBO J. 18:3924-3933, 1999). This report demonstrates by competitive index that the virulence phenotype of a spiC mutant is equivalent to that of a secretion component mutant. In addition, translocation of SPI2 effector proteins was shown to require SpiC. Thus, the severe virulence phenotype resulting from deletion of spiC is likely due to the inability to translocate all SPI2 effectors. SpiC was also required to secrete translocon proteins SseB and SseC but not translocated effector SseJ, indicating that lack of assembly of the translocon explains the spiC mutant phenotype.
引用
收藏
页码:4971 / 4980
页数:10
相关论文
共 43 条
[1]   pH-dependent secretion of SseB, a product of the SPI-2 type III secretion system of Salmonella typhimurium [J].
Beuzón, CR ;
Banks, G ;
Deiwick, J ;
Hensel, M ;
Holden, DW .
MOLECULAR MICROBIOLOGY, 1999, 33 (04) :806-816
[2]   Salmonella maintains the integrity of its intracellular vacuole through the action of SifA [J].
Beuzón, CR ;
Méresse, S ;
Unsworth, KE ;
Ruíz-Albert, J ;
Garvis, S ;
Waterman, SR ;
Ryder, TA ;
Boucrot, E ;
Holden, DW .
EMBO JOURNAL, 2000, 19 (13) :3235-3249
[3]   N-terminal conservation of putative type III secreted effectors of Salmonella typhimurium [J].
Brumell, JH ;
Marcus, SL ;
Finlay, BB .
MOLECULAR MICROBIOLOGY, 2000, 36 (03) :773-774
[4]   SifA permits survival and replication of Salmonella typhimurium in murine macrophages [J].
Brumell, JH ;
Rosenberger, CM ;
Gotto, GT ;
Marcus, SL ;
Finlay, BB .
CELLULAR MICROBIOLOGY, 2001, 3 (02) :75-84
[5]   A bacterial invasin induces macrophage apoptosis by binding directly to ICE [J].
Chen, YJ ;
Smith, MR ;
Thirumalai, K ;
Zychlinsky, A .
EMBO JOURNAL, 1996, 15 (15) :3853-3860
[6]   Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival [J].
Cirillo, DM ;
Valdivia, RH ;
Monack, DM ;
Falkow, S .
MOLECULAR MICROBIOLOGY, 1998, 30 (01) :175-188
[7]   The filamentous type III secretion translocon of enteropathogenic Escherichia coli [J].
Daniell, SJ ;
Takahashi, N ;
Wilson, R ;
Friedberg, D ;
Rosenshine, I ;
Booy, FP ;
Shaw, RK ;
Knutton, S ;
Frankel, G ;
Aizawa, S .
CELLULAR MICROBIOLOGY, 2001, 3 (12) :865-871
[8]   One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].
Datsenko, KA ;
Wanner, BL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645
[9]   Mutations in Salmonella pathogenicity island 2 (SP12) genes affecting transcription of SPI1 genes and resistance to antimicrobial agents [J].
Deiwick, J ;
Nikolaus, T ;
Shea, JE ;
Gleeson, C ;
Holden, DW ;
Hensel, M .
JOURNAL OF BACTERIOLOGY, 1998, 180 (18) :4775-4780
[10]   YopD of Yersinia pseudotuberculosis is translocated into the cytosol of HeLa epithelial cells:: evidence of a structural domain necessary for translocation [J].
Francis, MS ;
Wolf-Watz, H .
MOLECULAR MICROBIOLOGY, 1998, 29 (03) :799-813