High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor

被引：320

作者：

St-Pierre, Francois ^{[1
,2
]}

Marshall, Jesse D. ^{[3
,4
]}

Yang, Ying ^{[1
,2
]}

Gong, Yiyang ^{[3
,4
]}

Schnitzer, Mark J. ^{[3
,4
,5
]}

Lin, Michael Z. ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Pediat, Stanford, CA 94305 USA

[3] Stanford Univ, James H Clark Ctr, Stanford, CA 94305 USA

[4] Stanford Univ, CNC Program, Palo Alto, CA 94304 USA

[5] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

来源：

NATURE NEUROSCIENCE | 2014年 / 17卷 / 06期

基金：

美国国家科学基金会;

关键词：

MICROBIAL RHODOPSIN; ACTION-POTENTIALS; NEURAL ACTIVITY; PROTEIN; PROBE; MECHANISM; INTERNEURONS; INTEGRATION; INDICATORS; CIRCUITS;

D O I：

10.1038/nn.3709

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of similar to 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.

引用

页码：884 / 889

页数：6

共 34 条

[1] Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein [J].

Akemann, Walther ;

Mutoh, Hiroki ;

Perron, Amelie ;

Park, Yun Kyung ;

Iwamoto, Yuka ;

Knoepfel, Thomas .

JOURNAL OF NEUROPHYSIOLOGY, 2012, 108 (08) :2323-2337

[2] Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [J].

Akerboom, Jasper ;

Calderon, Nicole Carreras ;

Tian, Lin ;

Wabnig, Sebastian ;

Prigge, Matthias ;

Tolo, Johan ;

Gordus, Andrew ;

Orger, Michael B. ;

Severi, Kristen E. ;

Macklin, John J. ;

Patel, Ronak ;

Pulver, Stefan R. ;

Wardill, Trevor J. ;

Fischer, Elisabeth ;

Schueler, Christina ;

Chen, Tsai-Wen ;

Sarkisyan, Karen S. ;

Marvin, Jonathan S. ;

Bargmann, Cornelia I. ;

Kim, Douglas S. ;

Kugler, Sebastian ;

Lagnado, Leon ;

Hegemann, Peter ;

Gottschalk, Alexander ;

Schreiter, Eric R. ;

Looger, Loren L. .

FRONTIERS IN MOLECULAR NEUROSCIENCE, 2013, 6

[3] Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging [J].

Akerboom, Jasper ;

Chen, Tsai-Wen ;

Wardill, Trevor J. ;

Tian, Lin ;

Marvin, Jonathan S. ;

Mutlu, Sevinc ;

Calderon, Nicole Carreras ;

Esposti, Federico ;

Borghuis, Bart G. ;

Sun, Xiaonan Richard ;

Gordus, Andrew ;

Orger, Michael B. ;

Portugues, Ruben ;

Engert, Florian ;

Macklin, John J. ;

Filosa, Alessandro ;

Aggarwal, Aman ;

Kerr, Rex A. ;

Takagi, Ryousuke ;

Kracun, Sebastian ;

Shigetomi, Eiji ;

Khakh, Baljit S. ;

Baier, Herwig ;

Lagnado, Leon ;

Wang, Samuel S. -H. ;

Bargmann, Cornelia I. ;

Kimmel, Bruce E. ;

Jayaraman, Vivek ;

Svoboda, Karel ;

Kim, Douglas S. ;

Schreiter, Eric R. ;

Looger, Loren L. .

JOURNAL OF NEUROSCIENCE, 2012, 32 (40) :13819-13840

[4] A genetically targetable fluorescent probe of channel gating with rapid kinetics [J].

Ataka, K ;

Pieribone, VA .

BIOPHYSICAL JOURNAL, 2002, 82 (01) :509-516

[5] Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells [J].

Baker, B. J. ;

Lee, H. ;

Pieribone, V. A. ;

Cohen, L. B. ;

Isacoff, E. Y. ;

Knopfel, T. ;

Kosmidis, E. K. .

JOURNAL OF NEUROSCIENCE METHODS, 2007, 161 (01) :32-38

[6] A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials [J].

Barnett, Lauren ;

Platisa, Jelena ;

Popovic, Marko ;

Pieribone, Vincent A. ;

Hughes, Thomas .

PLOS ONE, 2012, 7 (09)

[7] Synaptic Integration Gradients in Single Cortical Pyramidal Cell Dendrites [J].

Branco, Tiago ;

Haeusser, Michael .

NEURON, 2011, 69 (05) :885-892

[8] Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein [J].

Cabantous, S ;

Terwilliger, TC ;

Waldo, GS .

NATURE BIOTECHNOLOGY, 2005, 23 (01) :102-107

[9] Genetically Targeted Optical Electrophysiology in Intact Neural Circuits [J].

Cao, Guan ;

Platisa, Jelena ;

Pieribone, Vincent A. ;

Raccuglia, Davide ;

Kunst, Michael ;

Nitabach, Michael N. .

CELL, 2013, 154 (04) :904-913

[10] Cortical Up and Activated States: Implications for Sensory Information Processing [J].

Castro-Alamancos, Manuel A. .

NEUROSCIENTIST, 2009, 15 (06) :625-634

← 1 2 3 4 →