Fluorescence enhancements on silver colloid coated surfaces

被引：57
作者：
Lukomska, J [1 ]
Malicka, J [1 ]
Gryczynski, I [1 ]
Lakowicz, JR [1 ]
机构：
[1] Univ Maryland, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA
来源：
JOURNAL OF FLUORESCENCE | 2004年 / 14卷 / 04期
关键词：
silver island films; colloids; metal-enhanced fluorescence;
D O I：
10.1023/B:JOFL.0000031823.04926.75
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.
引用
收藏
页码：417 / 423
页数：7
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