Intracellular trafficking and endocytic uptake pathway of Pepper vein banding virus-like particles in epithelial cells

被引：7

作者：

Sabharwal, Pallavi ^{[1
]}

Amritha, Chemmenchery K. ^{[1
]}

Sushmitha, Cheekati ^{[1
]}

Natraj, Usha ^{[1
]}

Savithri, Handanahal S. ^{[1
]}

机构：

[1] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India

来源：

NANOMEDICINE | 2019年 / 14卷 / 10期

关键词：

endocytosis; Hsp60; nanoparticles; subcellular targeting; vimentin; virus like particles (VLPs); CELLULAR UPTAKE; SURFACE VIMENTIN; COAT PROTEIN; POSTTRANSLATIONAL MODIFICATIONS; INTERMEDIATE-FILAMENTS; TOXICITY EVALUATION; ASPECT RATIO; NANOPARTICLES; CANCER; TUMOR;

D O I：

10.2217/nnm-2018-0405

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Plant virus-like particles (VLPs) have emerged as a novel platform for delivery of drugs/antibodies. The aim of the present investigation is to establish the entry mechanism of flexuous rod-shaped virus particles into mammalian cells. Methods: Far-Western blot analysis, pull-down and ELISA were used to characterize vimentin and Hsp60 interaction with VLPs. The mode/kinetics of internalization of VLPs was deciphered using pharmacological inhibitors/endosomal markers. Results & discussion: The flexuous rod-shaped VLPs of Pepper vein banding virus (PVBV) enter HeLa and HepG2 cells via cell-surface proteins: vimentin and Hsp60, respectively. VLPs internalize via different modes of endocytosis in HeLa, HepG2 cells and are biodegradable. Vimentin and Hsp60 could be potential epithelial ligands that facilitate targeting of nanoparticles to tumor cells.

引用

页码：1247 / 1265

页数：19

共 81 条

[51] Early Endosomal Escape of a Cyclic Cell-Penetrating Peptide Allows Effective Cytosolic Cargo Delivery [J].