Intracellular trafficking and endocytic uptake pathway of Pepper vein banding virus-like particles in epithelial cells

被引：7
作者：
Sabharwal, Pallavi [1 ]
Amritha, Chemmenchery K. [1 ]
Sushmitha, Cheekati [1 ]
Natraj, Usha [1 ]
Savithri, Handanahal S. [1 ]
机构：
[1] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India
来源：
NANOMEDICINE | 2019年 / 14卷 / 10期
关键词：
endocytosis; Hsp60; nanoparticles; subcellular targeting; vimentin; virus like particles (VLPs); CELLULAR UPTAKE; SURFACE VIMENTIN; COAT PROTEIN; POSTTRANSLATIONAL MODIFICATIONS; INTERMEDIATE-FILAMENTS; TOXICITY EVALUATION; ASPECT RATIO; NANOPARTICLES; CANCER; TUMOR;
D O I：
10.2217/nnm-2018-0405
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: Plant virus-like particles (VLPs) have emerged as a novel platform for delivery of drugs/antibodies. The aim of the present investigation is to establish the entry mechanism of flexuous rod-shaped virus particles into mammalian cells. Methods: Far-Western blot analysis, pull-down and ELISA were used to characterize vimentin and Hsp60 interaction with VLPs. The mode/kinetics of internalization of VLPs was deciphered using pharmacological inhibitors/endosomal markers. Results & discussion: The flexuous rod-shaped VLPs of Pepper vein banding virus (PVBV) enter HeLa and HepG2 cells via cell-surface proteins: vimentin and Hsp60, respectively. VLPs internalize via different modes of endocytosis in HeLa, HepG2 cells and are biodegradable. Vimentin and Hsp60 could be potential epithelial ligands that facilitate targeting of nanoparticles to tumor cells.
引用
收藏
页码：1247 / 1265
页数：19
相关论文
共 81 条
[1]   Intracellular delivery of antibodies by chimeric Sesbania mosaic virus (SeMV) virus like particles [J].
Abraham, Ambily ;
Natraj, Usha ;
Karande, Anjali A. ;
Gulati, Ashutosh ;
Murthy, Mathur R. N. ;
Murugesan, Sathyabalan ;
Mukunda, Pavithra ;
Savithri, Handanahal S. .
SCIENTIFIC REPORTS, 2016, 6
[2]   Surface-exposed amino- and carboxy-terminal residues are crucial for the initiation of assembly in Pepper vein banding virus:: a flexuous rod-shaped virus [J].
Anindya, R ;
Savithri, HS .
VIROLOGY, 2003, 316 (02) :325-336
[3]   Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns [J].
Bachmann, Martin F. ;
Jennings, Gary T. .
NATURE REVIEWS IMMUNOLOGY, 2010, 10 (11) :787-796
[4]   Cellular uptake of nanoparticles: journey inside the cell [J].
Behzadi, Shahed ;
Serpooshan, Vahid ;
Tao, Wei ;
Hamaly, Majd A. ;
Alkawareek, Mahmoud Y. ;
Dreaden, Erik C. ;
Brown, Dennis ;
Alkilany, Alaaldin M. ;
Farokhzad, Omid C. ;
Mahmoudi, Morteza .
CHEMICAL SOCIETY REVIEWS, 2017, 46 (14) :4218-4244
[5]   Nocodazole, a microtubule depolymerising agent, induces apoptosis of chronic lymphocytic leukaemia cells associated with changes in Bcl-2 phosphorylation and expression [J].
Beswick, RW ;
Ambrose, HE ;
Wagner, SD .
LEUKEMIA RESEARCH, 2006, 30 (04) :427-436
[6]   In vitro and in vivo toxicity evaluation of plant virus nanocarriers [J].
Blandino, Agnese ;
Lico, Chiara ;
Baschieri, Selene ;
Barberini, Lanfranco ;
Cirotto, Carlo ;
Blasi, Paolo ;
Santi, Luca .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2015, 129 :130-136
[7]  
Bohdan J, 1997, CELL BIOL INT, V21, P315
[8]   Biodistribution, pharmacokinetics, and blood compatibility of native and PEGylated tobacco mosaic virus nano-rods and -spheres in mice [J].
Bruckman, Michael A. ;
Randolph, Lauren N. ;
VanMeter, Allen ;
Hern, Stephen ;
Shoffstall, Andrew J. ;
Taurog, Rebecca E. ;
Steinmetz, Nicole F. .
VIROLOGY, 2014, 449 :163-173
[9]   Heterologous expression of the modified coat protein of Cowpea chlorotic mottle bromovirus results in the assembly of protein cages with altered architectures and function [J].
Brumfield, S ;
Willits, D ;
Tang, L ;
Johnson, JE ;
Douglas, T ;
Young, M .
JOURNAL OF GENERAL VIROLOGY, 2004, 85 :1049-1053
[10]   The Odyssey of Hsp60 from Tumor Cells to Other Destinations Includes Plasma Membrane-Associated Stages and Golgi and Exosomal Protein-Trafficking Modalities [J].
Campanella, Claudia ;
Bucchieri, Fabio ;
Merendino, Anna M. ;
Fucarino, Alberto ;
Burgio, Giosalba ;
Corona, Davide F. V. ;
Barbieri, Giovanna ;
David, Sabrina ;
Farina, Felicia ;
Zummo, Giovanni ;
de Macario, Everly Conway ;
Macario, Alberto J. L. ;
Cappello, Francesco .
PLOS ONE, 2012, 7 (07)
123456789