Large variation in t(11;14)(q13;q32) and t(14;18)(q32;q21) translocation product size is confirmed by sequence analysis of PCR products

被引：1

作者：

Wickham, C. L.

Harries, L. W.

Sarsfield, P.

Joyner, M. V.

Ellard, S.

机构：

[1] Royal Devon & Exeter NHS Fdn Trust, Dept Mol Genet, Exeter EX2 5DW, Devon, England

[2] Peninsula Med Sch, Inst Biomed & Clin Sci, Exeter, Devon, England

[3] Royal Devon & Exeter NHS Fdn Trust, Dept Histopathol, Exeter, Devon, England

[4] Royal Devon & Exeter NHS Fdn Trust, Dept Haematol, Exeter, Devon, England

来源：

CLINICAL AND LABORATORY HAEMATOLOGY | 2006年 / 28卷 / 04期

关键词：

t(11; 14)(q13; q32); t(14; 18)(q32; q21); chromosomal translocation; sequencing;

D O I：

10.1111/j.1365-2257.2006.00790.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Polymerase chain reaction is commonly used to detect t(11;14)(q13;q32) and t(14;18)(q32;q21) chromosomal translocations associated with mantle cell lymphoma and follicular lymphoma. We tested a total of 482 samples from patients with suspected non-Hodgkin's lymphoma and sequenced unusual-sized t(11;14)(q13;q32) and t(14;18)(q32;q21) products from 33 of these patients. BCL-1 or BCL-2 gene rearrangements were confirmed in 23 of 33 patients (70%). Considerable size variation was observed using t(11;14) primers, with MTCA and MTCB t(11;14) products ranging from 234 to 934 bp and 143 to 560 bp respectively. Less variability was observed for t(14;18) Major Breakpoint Region (MBR) products (100-252 bp) but Minor Cluster Region (MCR) products ranged from 217 to 498 bp. We demonstrate the utility of sequence analysis to confirm unusual-sized translocation products and reduce false-positive results because of nonspecific amplification.

引用

页码：248 / 253

页数：6

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