Quantitative analysis of C-60 fullerene in blood and tissues by high-performance liquid chromatography with photodiode-array and mass spectrometric detection

被引：46

作者：

Moussa, F

Pressac, M

Genin, E

Roux, S

Trivin, F

Rassat, A

Ceolin, R

Szwarc, H

机构：

[1] FAC PHARM, SERV ENDOCRINOL, F-75006 PARIS, FRANCE

[2] FINNIGAN MAT SARL, F-91893 ORSAY, FRANCE

[3] ECOLE NORMALE SUPER, URA 1110 CNRS, F-75 PARIS, FRANCE

[4] UNIV PARIS 11, URA D1104 CNRS, F-91405 ORSAY, FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 696卷 / 01期

关键词：

C-60; fullerene;

D O I：

10.1016/S0378-4347(97)00228-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) assay method for C-60 fullerene, in blood, liver and spleen using photodiode-array detection or mass spectrometric detection (LC-MS) and C-70 fullerene, as the internal standard, is described. The recovery from mouse blood and tissues spilled with micronized C-60 exceeds 90%. The method is linear from 0.05 to 200 mg of C-60 per liter of blood and from 0.05 to 5.00% of C-60 per tissue weight, The limit of detection of the method is 0.1 ng of C-60 per injection, This method was applied to mouse blood and tissue samples after intraperitoneal administration of a micronized C-60 suspension. (C) 1997 Elsevier Science B.V.

引用

页码：153 / 159

页数：7

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