In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells

被引：135

作者：

Gang, EJ ^{[1
]}

Hong, SH ^{[1
]}

Jeong, JA ^{[1
]}

Hwang, SH ^{[1
]}

Kim, SW ^{[1
]}

Yang, IH ^{[1
]}

Ahn, CY ^{[1
]}

Han, H ^{[1
]}

Kim, H ^{[1
]}

机构：

[1] Histostem Co, Biotechnol Res Inst, Seoul, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 321卷 / 01期

关键词：

umbilical cord blood; mesenchymal stem cells; mesengenic differentiation osteoblast; chondrocyte; adipocyte; skeletal myoblast;

D O I：

10.1016/j.bbrc.2004.06.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human mesenchymal stem cells (hMSCs) have been paid a great deal of attention because of their unprecedented therapeutic merits endowed by powerful ex vivo expansion and multilineage differentiation potential. Umbilical cord blood (UCB) is a convenient but not fully proven source for hMSCs, and hence, greater experience is required to establish UCB as a reliable source of hMSCs. To this end, we attempted to isolate hMSC-like adherent cells from human UCB. The isolated cells were highly proliferative and exhibited an immunophenotype of CD13(+) CD14(-) CD29(+) CD31(-) CD34(-) CD44(+) CD45(-) CD49e(+) CD54(+) CD90(+) CD106(-) ASMA(+) SH2(+) SH3(+) HLA-ABC(+) HLA-DR-. More importantly, these cells, under appropriate conditions, could differentiate into a variety of mesenchymal lineage cells such as osteoblasts, chondrocytes, adipocytes, and skeletal myoblasts. This mesengenic potential assures that the UCB-derived cells are multipotent hMSCs and further implicates that UCB can be a legitimate source of hMSCs. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：102 / 108

页数：7

共 25 条

[1] Therapeutic angiogenesis using autologous bone marrow stromal cells: Improved blood flow in a chronic limb ischemia model [J].