Fluorescent Cassettes for Monitoring Three-Component Interactions in Vitro and in Living Cells

被引：45

作者：

Wu, Liangxing ^{[1
]}

Loudet, Aurore ^{[1
]}

Barhoumi, Rola ^{[2
]}

Burghardt, Robert C. ^{[2
]}

Burgess, Kevin ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77841 USA

[2] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77841 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 26期

关键词：

ENERGY-TRANSFER CASSETTES; PROTEIN;

D O I：

10.1021/ja9029413

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two water-soluble "through-bond energy transfer cassettes" (TBET-cassettes) were prepared. They have good extinction coefficients at the donor part and transfer energy to the acceptor parts with good "overall quantum yields" (0.30 and 0.24 in pH 7.4 phosphate buffer). Fluorescence resonance energy transfer from one protein functionalized with an appropriate donor can be used to excite the TBET-cassettes on different proteins to probe protein-protein interactions under conditions that would not be possible for single-dye acceptor systems.

引用

页码：9156 / +

页数：3

共 9 条

[1] Water-soluble through-bond energy transfer cassettes for intracellular imaging [J].

Bandichhor, Rakeshwar ;

Petrescu, Anca D. ;

Vespa, Aude ;

Kier, Ann B. ;

Schroeder, Friedhelm ;

Burgess, Kevin .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (33) :10688-10689

[2] 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) dyes modified for extended conjugation and restricted bond rotations [J].

Chen, J ;

Burghart, A ;

Derecskei-Kovacs, A ;

Burgess, K .

JOURNAL OF ORGANIC CHEMISTRY, 2000, 65 (10) :2900-2906

[3] Quantitative photoelectrochemical detection of biological affinity reaction: Biotin-avidin interaction [J].

Dong, D ;

Zheng, D ;

Wang, FQ ;

Yang, XQ ;

Wang, N ;

Li, YG ;

Guo, LH ;

Cheng, J .

ANALYTICAL CHEMISTRY, 2004, 76 (02) :499-501

[4] Fluorescent, through-bond energy transfer cassettes for Labeling multiple biological molecules in one experiment [J].

Jiao, GS ;

Thoresen, LH ;

Burgess, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14668-14669

[5] Syntheses and spectral properties of functionalized, water-soluble BODIPY derivatives [J].

Li, Lingling ;

Han, Junyan ;

Nguyen, Binh ;

Burgess, Kevin .

JOURNAL OF ORGANIC CHEMISTRY, 2008, 73 (05) :1963-1970

[6] Cu-catalyzed azide-alkyne cycloaddition [J].

Meldal, Morten ;

Tornoe, Christian Wenzel .

CHEMICAL REVIEWS, 2008, 108 (08) :2952-3015

[7] Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein [J].

Miyawaki, A ;

Tsien, RY .

APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS PT B: CELL BIOLOGY AND PHYSIOLOGY, 2000, 327 :472-500

[8] A peptide carrier for the delivery of biologically active proteins into mammalian cells [J].

Morris, MC ;

Depollier, J ;

Mery, J ;

Heitz, F ;

Divita, G .

NATURE BIOTECHNOLOGY, 2001, 19 (12) :1173-1176

[9] Analysis of protein interactions using fluorescence technologies [J].

Yan, YL ;

Marriott, G .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2003, 7 (05) :635-640

← 1 →