Cloning and over-expression of an alkaline protease from Bacillus licheniformis

被引：27
|
作者：
Tang, XM
Shen, W
Lakay, FM
Shao, WL
Wang, ZX
Prior, BA
Zhuge, J
机构：
[1] Univ Stellenbosch, Dept Microbiol, ZA-7602 Matieland, South Africa
[2] So Yangtze Univ, Res Ctr Ind Microbiol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214036, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 12期
关键词：
alkaline protease; Bacillus licheniformis; over-expression; pro-sequence; subtilisin;
D O I：
10.1023/B:BILE.0000030042.91094.38
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The alkaline protease gene, apr, from Bacillus licheniformis 2709 was cloned into a Bacillus shuttle expression vector, pHL, to yield the recombinant plasmid pHL-apr. The pHL-apr was expressed in Bacillus subtilis WB600, yielding a high expression strain BW-016. The amount of alkaline protease produced in the recombinant increased by 65% relative to the original strain. SDS-PAGE analysis indicated a Mr of 30.5 kDa. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 6816.
引用
收藏
页码：975 / 979
页数：5
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