The design and synthesis of bis-[4′-Azido-2,2′:6′,2"-terpyridine platinum(II)] complexes with rigid and extended linkers for studying the topology of DNA by photoaffinity labeling

被引：37

作者：

Lowe, G ^{[1
]}

Droz, AS ^{[1
]}

Park, JJ ^{[1
]}

Weaver, GW ^{[1
]}

机构：

[1] Univ Oxford, Dyson Perrins Lab, Oxford OX1 3QY, England

来源：

BIOORGANIC CHEMISTRY | 1999年 / 27卷 / 06期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

bis-intercalators; photoaffinity labeling; molecular rulers; DNA topology;

D O I：

10.1006/bioo.1999.1152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of bis-[4'-Azido-2,2':6',2 "-terpyridine platinum(II)] complexes with linear Linkers of varying length have been synthesized. They have been designed to bis-intercalate into two DNA duplexes in close proximity, the azido groups allowing the sites of intercalation to be photoaffinity labeled. The linker to Pt(II) bonds are susceptible to cleavage by thiols and cyanide ion, which is a requirement for the intended method of analysis by 2D gel electrophoresis. (C) 1999 Academic Press.

引用

页码：477 / 486

页数：10

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