A fully portable microchip real-time polymerase chain reaction for rapid detection of pathogen

被引：21

作者：

Tong, Rui ^{[1
]}

Zhang, Lijuan ^{[2
]}

Song, Qi ^{[3
]}

Hu, Chuandeng ^{[4
]}

Chen, Xuee ^{[1
]}

Lou, Kai ^{[2
]}

Gong, Xiuqing ^{[2
]}

Gao, Yibo ^{[2
]}

Wen, Weijia ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Nano Sci & Technol NSNT Program, Kowloon, Clear Water Bay, Hong Kong, Peoples R China

[2] Shenzhen Shineway Hitech, Shenzhen, Peoples R China

[3] Guangzhou HKUST Fok Ying Tung Res Inst, Guangzhou, Guangdong, Peoples R China

[4] Hong Kong Univ Sci & Technol, Dept Phys, Kowloon, Clear Water Bay, Hong Kong, Peoples R China

来源：

ELECTROPHORESIS | 2019年 / 40卷 / 12-13期

关键词：

DNA; Microchip real-time PCR; Pathogen; Point-of-care testing; PCR; EBOLA; CHIP; VIRUS;

D O I：

10.1002/elps.201900090

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Point-of-care detection for pathogen is of critical need for wide epidemic warning and medical diagnosis. In this work, we have designed and developed a fully portable and integrated microchip based real-time polymerase chain reaction machine for rapid pathogen detection. The instrument consists of three functional components including heating, optical, and electrical modules, which are integrated into a portable compact box. The microchip is consumable material replaceable to meet various detection needs. Consequently, we demonstrated the outstanding performance of this portable machine for rapid detection of Salmonella and Escherichia coli O157:H7 with the advantage of time-saving (similar to 25 min), less samples consumption, portability, and user-friendly operation.

引用

页码：1699 / 1707

页数：9

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