Chromosomal DNA deletion confers phage resistance to Pseudomonas aeruginosa

被引：86

作者：

Le, Shuai ^{[1
,2
]}

Yao, Xinyue ^{[1
]}

Lu, Shuguang ^{[1
]}

Tan, Yinling ^{[1
]}

Rao, Xiancai ^{[1
]}

Li, Ming ^{[1
]}

Jin, Xiaolin ^{[1
]}

Wang, Jing ^{[1
]}

Zhao, Yan ^{[1
]}

Wu, Nicholas C. ^{[3
]}

Lux, Renate ^{[2
]}

He, Xuesong ^{[2
]}

Shi, Wenyuan ^{[2
]}

Hu, Fuquan ^{[1
]}

机构：

[1] Third Mil Med Univ, Dept Microbiol, Chongqing 400038, Peoples R China

[2] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

基金：

中国国家自然科学基金;

关键词：

BACTERIOPHAGE THERAPY; CAMPYLOBACTER-JEJUNI; GENOME; ADAPTATION; INFECTION; SYSTEM; GENE; GALU; LIPOPOLYSACCHARIDE; COEVOLUTION;

D O I：

10.1038/srep04738

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacteria develop a broad range of phage resistance mechanisms, such as prevention of phage adsorption and CRISPR/Cas system, to survive phage predation. In this study, Pseudomonas aeruginosa PA1 strain was infected with lytic phage PaP1, and phage-resistant mutants were selected. A high percentage (similar to 30%) of these mutants displayed red pigmentation phenotype (Red mutant). Through comparative genomic analysis, one Red mutant PA1r was found to have a 219.6 kb genomic fragment deletion, which contains two key genes hmgA and galU related to the observed phenotypes. Deletion of hmgA resulted in the accumulation of a red compound homogentisic acid; while A galU mutant is devoid of O-antigen, which is required for phage adsorption. Intriguingly, while the loss of galU conferred phage resistance, it significantly attenuated PA1r in a mouse infection experiment. Our study revealed a novel phage resistance mechanism via chromosomal DNA deletion in P. aeruginosa.

引用

页数：8

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