Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens

被引：20

作者：

Ha, Suk-Jin ^{[1
,2
]}

Kim, Sang-Yong ^{[1
]}

Seo, Jin-Ho ^{[2
]}

Jeya, Marimuthu ^{[3
]}

Zhang, Ye-Wang ^{[3
]}

Ramu, Thangadurai ^{[3
]}

Kim, In-Won ^{[3
]}

Lee, Jung-Kul ^{[3
]}

机构：

[1] BioNgene Co Ltd, Seoul 110521, South Korea

[2] Seoul Natl Univ, Dept Biosci & Biotechnol, Seoul 151742, South Korea

[3] Konkuk Univ, Dept Chem Engn, Seoul 143701, South Korea

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2009年 / 32卷 / 05期

关键词：

Agrobacterium tumefaciens; Ca2+; Coenzyme Q(10); Oxidative stress; DECAPRENYL DIPHOSPHATE SYNTHASE; CAROTENOID BIOSYNTHESIS; GENE; CLONING; OPTIMIZATION; MITOCHONDRIA; UBIQUINONE; OXYGEN;

D O I：

10.1007/s00449-009-0318-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Of various metal ions (Ca2+, Cr3+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+ and Zn2+) added to the culture medium of Agrobacterium tumefaciens at 1 mM, only Ca2+ increased Coenzyme Q10 (CoQ(10)) content in cells without the inhibition of cell growth. In a pH-stat fed-batch culture, supplementation with 40 mM of CaCO3 increased the specific CoQ(10) content and oxidative stress by 22.4 and 48%, respectively. Also, the effect of Ca2+ on the increase of CoQ(10) content was successfully verified in a pilot-scale (300 L) fermentor. In this study, the increased oxidative stress in A. tumefaciens culture by the supplementation of Ca2+ is hypothesized to stimulate the increase of specific CoQ(10) content in order to protect the membrane against lipid peroxidation. Our results improve the understanding of Ca2+ effect on CoQ(10) biosynthesis in A. tumefaciens and should contribute to better industrial production of CoQ(10) by biological processes.

引用

页码：697 / 700

页数：4

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