Evaluating five dedicated automatic devices for haemoglobinopathy diagnostics in multi-ethnic populations

被引：104

作者：

Van Delft, P.

Lenters, E. ^{[2
]}

Bakker-Verweij, M.

de Korte, M.

Baylan, U.

Harteveld, C. L.

Giordano, P. C. ^{[1
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Human & Clin Genet, Haemoglobinopathies Lab,Ctr Human & Clin Genet, NL-2300 RC Leiden, Netherlands

[2] Isala Clin, Dept Clin Chem, Zwolle, Netherlands

来源：

INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2009年 / 31卷 / 05期

关键词：

HPLC; capillary electrophoresis; thalassaemia; sickle cell disease; diagnosis; A(2); VARIANTS; RETENTION; MUTATION; SAMPLES; SYSTEM;

D O I：

10.1111/j.1751-553X.2009.01158.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

P>We have tested five haemoglobin (Hb) separation apparatuses, dedicated to haemoglobinopathy diagnostics. These are the four high performance liquid chromatography devices: VARIANT II (TM), HA 8160, G7, Ultra(2) and the Capillary Electrophoresis apparatus from Sebia. In the first place, we focussed on the capacity of all apparatuses to detect the most common structural variants relevant for public health, these being HbS, HbC, HbE, HbD-Punjab and HbO-Arab. We then compared how the high HbA(2) beta-thalassaemia carriers were identified. All apparatuses were able to identify carriers of these traits with the expected sensitivity and specificity. With the primary goal of a high degree of conformity in basic diagnostics of haemoglobinopathies, we present the interpretation and the significance of the results on all apparatuses, and we comment on the unavoidable problems and solutions.

引用

页码：484 / 495

页数：12

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