Effects of osmolytes on unfolding of chicken liver fatty acid synthase

被引：9
|
作者：
Park, YD
Wu, BN
Tian, WX
Zhou, HM [1 ]
机构：
[1] Tsinghua Univ, Sch Life Sci & Engn, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Tsinghua Univ, Sch Life Sci & Engn, Minist Educ, Prot Sci Lab, Beijing 100084, Peoples R China
来源：
BIOCHEMISTRY-MOSCOW | 2002年 / 67卷 / 08期
基金：
中国国家自然科学基金;
关键词：
fatty acid synthase; urea denaturation; aggregation; osmolyte; artificial chaperone;
D O I：
10.1023/A:1019970721758
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Urea-induced aggregation of chicken liver fatty acid synthase [acyl-Coa:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl-and enoyl-reducing and thioester-hydrolyzing), EC 2.3.1.85] was studied. The aggregation was facilitated at increased ionic strength. Methyl-beta-cyclodextrin and some osmolytes, such as glycerol, sucrose, proline, glycine, and heparin, could effectively prevent the aggregation, implying an artificial chaperone role of those substances during fatty acid synthase unfolding. The osmolytes also protected the enzyme from inactivation.
引用
收藏
页码：914 / 917
页数：4
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