Glucansucrase Produced by Lactic Acid Bacteria: Structure, Properties, and Applications

被引：13

作者：

Yu, Liansheng ^{[1
,2
,3
,4
]}

Qian, Zhigang ^{[5
]}

Ge, Jingping ^{[1
,2
,3
,4
]}

Du, Renpeng ^{[1
,2
,3
,4
,5
]}

机构：

[1] Heilongjiang Univ, Engn Res Ctr Agr Microbiol Technol, Minist Educ, Harbin 150080, Peoples R China

[2] Heilongjiang Univ, Heilongjiang Prov Key Lab Plant Genet Engn & Biol, Harbin 150080, Peoples R China

[3] Heilongjiang Univ, Coll Heilongjiang Prov, Key Lab Microbiol, Harbin 150080, Peoples R China

[4] Heilongjiang Univ, Sch Life Sci, Harbin 150080, Peoples R China

[5] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

来源：

FERMENTATION-BASEL | 2022年 / 8卷 / 11期

基金：

中国博士后科学基金;

关键词：

lactic acid bacteria; glucansucrase; exopolysaccharides; structure and properties; applications; MESENTEROIDES NRRL B-512F; LEUCONOSTOC-MESENTEROIDES; MOLECULAR CHARACTERIZATION; DEXTRANSUCRASE GENE; SEQUENCE-ANALYSIS; IN-VITRO; 4,6-ALPHA-GLUCANOTRANSFERASE ENZYMES; BIOCHEMICAL-CHARACTERIZATION; NUCLEOTIDE-SEQUENCE; EXPRESSION ANALYSIS;

D O I：

10.3390/fermentation8110629

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucansucrase (GS) belongs to the GH70 family, which not only can synthesize exopolysaccharides (EPSs) with different physicochemical properties through glucosyl transglycosylation (by hydrolyzing sucrose) but can also produce oligosaccharides. Different strains produce different GSs, which catalyze the synthesis of EPS with different glycosidic bond structures; these EPSs have different biological functions. As an important enzymatic tool, GS has great potential in health care medicine, biological materials, ecological protection, the food chemical industry, etc. GS is mainly produced by lactic acid bacteria (LAB), including Leuconostoc, Streptococcus, Lactobacillus, and Weissella species. With the elucidation of the crystal structure of GS and the advancement of genome sequencing technology, its synthesis reaction mechanism and specific structural characteristics are gradually becoming clear. This review summarizes the isolation, purification, physical and chemical properties, detection methods, sources, and applications of GS in order to provide a reference for the research and development of GS.

引用

页数：20