Protection against oxidative damage of erythrocyte membrane by the flavonoid quercetin and its relation to iron chelating activity

被引：283

作者：

Ferrali, M ^{[1
]}

Signorini, C ^{[1
]}

Caciotti, B ^{[1
]}

Sugherini, L ^{[1
]}

Ciccoli, L ^{[1
]}

Giachetti, D ^{[1
]}

Comporti, M ^{[1
]}

机构：

[1] UNIV SIENA,INST GEN PATHOL,I-53100 SIENA,ITALY

来源：

FEBS LETTERS | 1997年 / 416卷 / 02期

关键词：

erythrocytes; oxidizing agent; quercetin; iron chelation; lipid peroxidation; hemolysis; senescent cell antigen;

D O I：

10.1016/S0014-5793(97)01182-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of glutathione (GSH) depleted mouse erythrocytes with the oxidants phenylhydrazine, acrolein, divicine and isouramil resulted in the release of free iron and in lipid peroxidation and hemolysis. The addition of the flavonoid quercetin, which chelates iron and penetrates erythrocytes, resulted in remarkable protection against lipid peroxidation and hemolysis. The protection seems to be due to intracellular chelation of iron, since a semi-stoichiometric ratio between released iron and the amount of quercetin necessary to prevent lipid peroxidation and hemolysis was found, Incubation of GSH depleted human erythrocytes with divicine and isouramil did not induce lipid peroxidation and hemolysis in spite of a substantial release of iron. However, divicine and isouramil produced alterations of membrane proteins, such as spectrin and band 3, as web as formation of senescent cell antigen, The addition of quercetin prevented these alterations. (C) 1997 Federation of European Biochemical Societies.

引用

页码：123 / 129

页数：7

共 42 条

[1] DIFFERENT ANTIOXIDANT ACTIVITIES OF BIOFLAVONOID RUTIN IN NORMAL AND IRON-OVERLOADING RATS [J].