Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato

被引：72

作者：

Ben Khedher, Saoussen ^{[1
]}

Kilani-Feki, Olfa ^{[1
,2
]}

Dammak, Mouna ^{[1
]}

Jabnoun-Khiareddine, Hayfa ^{[3
]}

Daami-Remadi, Mejda ^{[3
]}

Tounsi, Slim ^{[1
]}

机构：

[1] Univ Sfax, Ctr Biotechnol Sfax, Lab Biopesticides, Sfax 3018, Tunisia

[2] Ecole Polytech Sousse, Dept Genie Biotechnol, Sousse 4045, Tunisia

[3] Univ Sousse, Reg Ctr Res Hort & Organ Agr ChottMariem, Integrated Hort Prod Tunisian Ctr East, UR13AGR09, Chott Mariem 4042, Tunisia

来源：

COMPTES RENDUS BIOLOGIES | 2015年 / 338卷 / 12期

关键词：

Biological control; Bacillus subtilis; Rhizoctonia solani; Antifungal activity; Potato tuber; SHEATH BLIGHT; VERTICILLIUM-BIGUTTATUM; BLACK SCURF; BACTERIA; BIOCONTROL; RICE; RHIZOSPHERE; STRAINS; YIELD; KUHN;

D O I：

10.1016/j.crvi.2015.09.005

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study is to evaluate the efficacy of the strain Bacillus subtilis V26, a local isolate from the Tunisian soil, to control potato black scurf caused by Rhizoctonia solani. The in vitro antifungal activity of V26 significantly inhibited R. solani growth compared to the untreated control. Microscopic observations revealed that V26 caused considerable morphological deformations of the fungal hyphae such as vacuolation, protoplast leakage and mycelia crack. The most effective control was achieved when strain V26 was applied 24 h prior to inoculation (protective activity) in potato slices. The antagonistic bacterium V26 induced significant suppression of root canker and black scurf tuber colonization compared to untreated controls with a decrease in incidence disease of 63% and 81%, respectively, and promoted plant growth under greenhouse conditions on potato plants. Therefore, B. subtilis V26 has a great potential to be commercialized as a biocontrol agent against R. solani on potato crops. (C) 2015 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：784 / 792

页数：9

共 46 条

[1]

Banville G.J., 1978, American Potato Journal, V55, P56

[2] YIELD LOSSES AND DAMAGE TO POTATO PLANTS CAUSED BY RHIZOCTONIA-SOLANI KUHN [J].

BANVILLE, GJ .

AMERICAN POTATO JOURNAL, 1989, 66 (12) :821-834

[3] Efficacy of several potential biocontrol organisms against Rhizoctonia solani on potato [J].

Brewer, MT ;

Larkin, RP .

CROP PROTECTION, 2005, 24 (11) :939-950

[4] SYMPTOMS, SIGNS AND YIELD REDUCTION ASSOCIATED WITH RHIZOCTONIA DISEASE OF POTATO INDUCED BY TUBER-BORNE INOCULUM OF RHIZOCTONIA-SOLANI AG-3 [J].

CARLING, DE ;

LEINER, RH ;

WESTPHALE, PC .

AMERICAN POTATO JOURNAL, 1989, 66 (11) :693-701

[5] Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level [J].

Cavaglieri, L ;

Orlando, J ;

Rodríguez, MI ;

Chulze, S ;

Etcheverry, M .

RESEARCH IN MICROBIOLOGY, 2005, 156 (5-6) :748-754

[6] Characteristics of proteinases and lipases produced by seven Bacillus sp isolated from milk powder production lines [J].

Chen, L ;

Coolbear, T ;

Daniel, RM .

INTERNATIONAL DAIRY JOURNAL, 2004, 14 (06) :495-504

[7]

Daami-Remadi M., 2008, Afr J Plant Sci Biotechnol, V2, P34

[8]

Daami-Remadi M., 2008, The African Journal of Plant Science and Biotechnology, V2, P57

[9] Integrated management strategies of bean root rot with Bacillus subtilis and Rhizobium in Minnesota [J].

Estevez de Jensen, Consuelo ;

Percich, J.A. ;

Graham, P.H. .

2002, Elsevier (74) :2-3

[10]

Demirci E, 2009, AFR J BIOTECHNOL, V8, P2503

← 1 2 3 4 5 →