Reactive oxygen species damaged human serum albumin in patients with type 1 diabetes mellitus: Biochemical and immunological studies

被引:59
|
作者
Rasheed, Zafar [1 ]
Ali, Rashid [1 ]
机构
[1] Aligarh Muslim Univ, JN Med Coll, Fac Med, Dept Biochem, Aligarh 202002, Uttar Pradesh, India
关键词
HSA; ROS-HSA; type 1 diabetes mellitus; reactive oxygen species;
D O I
10.1016/j.lfs.2006.07.041
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The role of hydroxyl radical (OH) damaged human serum albumin (HSA) in type I diabetes has been investigated in the present study. Hydroxyl radical induced modification on HSA has been studied by UV absorption spectroscopy, ANS fluorescence and carbonyl estimation. Hydroxyl radical modified HSA was found to be highly immunogenic in rabbits as compared to native HSA. The binding characteristics of circulating autoantibodies in type I diabetes patients against native and modified HSA were assessed. Diabetes patients (n=31) were examined by direct binding ELISA and the results were compared with healthy age-matched controls (n=22). High degree of specific binding by 54.8% of patients sera towards OH modified HSA, in comparison to its native analogue (p < 0.05) was observed. Sera from those type 1 diabetes patients having smoking history, high aging with high degree of disease showed substantially stronger binding to OH modified HSA over native HSA in particular. Normal human sera showed negligible binding with either antigen. Competitive inhibition ELISA reiterates the direct binding results. Gel retardation assay further substantiated the enhanced recognition of modified HSA by circulating autoantibodies in diabetes patients. The increase in total serum protein carbonyl levels in the diabetes patients was largely due to an increase in oxidized albumin. HSA of diabetes mellitus patients (DM-HSA) and normal subjects (normal-HSA) were purified on a Sephacryl S-200 HR column. Spectroscopic analysis confirmed that the DM-HSA samples contained higher levels of carbonyls than normal-HSA (p < 0.001). DM-HSA was conformationally altered, with more exposure of its hydrophobic regions. Collectively, the oxidation of plasma proteins, especially HSA, might enhance oxidative stress in type I diabetes mellitus patients. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:2320 / 2328
页数:9
相关论文
共 50 条
  • [1] Reactive oxygen species damaged human serum albumin in patients with hepatocellular carcinoma
    Rasheed, Z.
    Ahmad, R.
    Rasheed, N.
    Ali, R.
    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2007, 26 (03) : 395 - 404
  • [2] Enhanced recognition of reactive oxygen species damaged human serum albumin by circulating systemic lupus erythematosus autoantibodies
    Sheikh, Zafar
    Ahmad, Rizwan
    Sheikh, Naila
    Ali, Rashid
    AUTOIMMUNITY, 2007, 40 (07) : 512 - 520
  • [3] Mitochondrial Reactive Oxygen Species and Type 1 Diabetes
    Chen, Jing
    Stimpson, Scott E.
    Fernandez-Bueno, Gabriel A.
    Mathews, Clayton E.
    ANTIOXIDANTS & REDOX SIGNALING, 2018, 29 (14) : 1361 - 1372
  • [4] Reactive oxygen species, reactive nitrogen species and antioxidants in etiopathogenesis of diabetes mellitus type-2
    P. P. Singh
    Farzana Mahadi
    Ajanta Roy
    Praveen Sharma
    Indian Journal of Clinical Biochemistry, 2009, 24 (4) : 324 - 342
  • [5] REACTIVE OXYGEN SPECIES, REACTIVE NITROGEN SPECIES AND ANTIOXIDANTS IN ETIOPATHOGENESIS OF DIABETES MELLITUS TYPE-2
    Singh, P. P.
    Mahadi, Farzana
    Roy, Ajanta
    Sharma, Praveen
    INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY, 2009, 24 (04) : 324 - 342
  • [6] Immunological Studies of Reactive Oxygen Species Damaged Catalase in Patients with Systemic Lupus Erythematosus: Correlation with Disease Activity Index
    Al-Shobaili, Hani A.
    Al Robaee, Ahmad A.
    Alzolibani, Abdullateef A.
    Rasheed, Zafar
    IMMUNOLOGICAL INVESTIGATIONS, 2013, 42 (03) : 191 - 203
  • [7] Cys34 Adducts of Reactive Oxygen Species in Human Serum Albumin
    Grigoryan, Hasmik
    Li, He
    Iavarone, Anthony T.
    Williams, Evan R.
    Rappaport, Stephen M.
    CHEMICAL RESEARCH IN TOXICOLOGY, 2012, 25 (08) : 1633 - 1642
  • [8] Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus
    Signorello, M. G.
    Viviani, G. L.
    Armani, U.
    Cerone, R.
    Minniti, G.
    Piana, A.
    Leoncini, G.
    THROMBOSIS RESEARCH, 2007, 120 (04) : 607 - 613
  • [9] Structural and immunological characterization of Amadori-rich human serum albumin: Role in diabetes mellitus
    Arif, Binish
    Ashraf, Jalaluddin M.
    Moinuddin
    Ahmad, Jamal
    Arif, Zarina
    Alam, Khursheed
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2012, 522 (01) : 17 - 25
  • [10] Preserved coronary arteriolar dilatation in patients with type 2 diabetes mellitus: Implications for reactive oxygen species
    Zsolt Bagi
    Attila Feher
    Timea Beleznai
    Pharmacological Reports, 2009, 61 : 99 - 104