Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation

被引：17

作者：

Alexander, Marina R. ^{[1
]}

Rootes, Christina L. ^{[1
]}

van Vuren, Petrus Jansen ^{[1
]}

Stewart, Cameron R. ^{[1
]}

机构：

[1] CSIRO Hlth & Biosecur, Australian Ctr Dis Preparedness, Private Bag 24, Geelong, Vic 3220, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2020年 / 286卷

关键词：

SARS-CoV-2; COVID-19; PEG precipitation; Virus concentration; VIRUS; PURIFICATION;

D O I：

10.1016/j.jviromet.2020.113977

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a similar to 1.5 log(10) increase in SARS-CoV-2 titres of virus prepared in VeroE6 cells and enabled the infection of several immortalized human cell lines (Caco-2 and Calu-3) at a high multiplicity of infection not practically achievable without virus concentration. This protocol underscores the utility of PEG-mediated precipitation for SARS-CoV-2 and provides a resource for a range of coronavirus research areas.

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