LncRNA OIP5-AS1 Promotes Breast Cancer Progression by Regulating miR-216a-5p/GLO1

被引:34
作者
Wu, Zizheng [1 ]
Liu, Yinfeng [1 ]
Wei, Liguang [1 ]
Han, Meng [1 ]
机构
[1] First Hosp Qinhuangdao, Dept Breast Surg, 258 Wenhua Rd, Qinhuangdao 066000, Hebei, Peoples R China
关键词
OIP5-AS1; miR-216a-5p; GLO1; Breast cancer; GLYOXALASE; 1; PROLIFERATION; CELLS; METASTASIS; EXPRESSION; METHYLGLYOXAL; ONCOGENE;
D O I
10.1016/j.jss.2020.07.067
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Breast cancer is a familiar malignant tumor, which is a great threat to women's life. Long noncoding RNA Opa interacting protein 5-antisense RNA 1 (OIP5-AS1) has been reported to be associated with numerous cancers. This study aimed to explore the role of OIP5-AS1 and the mechanism of its action in the progression of breast cancer. Methods: The expression of OIP5-AS1 and miR-216a-5p was detected by quantitative realtime polymerase chain reaction. Cell proliferation, apoptosis, migration, or invasion was assessed by 4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, flow cytometry, or transwell assay, respectively. The binding sites were predicted by bioinformatics tool starBase2.0 (http://starbase.sysu.edu.cn/starbase2/index.php). The interaction between miR-216a-5p and OIP5-AS1 or glyoxalase 1 (GLO1) was confirmed by dual-luciferase reporter assay. The expression of GLO1 was quantified by Western blot. Nude mouse tumorigenicity assays were conducted to verify the role of OIP5-AS1 in vivo. Results: OIP5-AS1 and GLO1 were highly expressed in both clinical tumor tissues and cell lines, whereas miR-216a-5p was downregulated. Knockdown of OIP5-AS1 suppressed proliferation, migration, and invasion but promoted apoptosis of breast cancer cells. MiR216a-5p was a target of OIP5-AS1 and interacted with GLO1. MiR-216a-5p inhibition or GLO1 overexpression reversed the effects of OIP5-AS1 knockdown on the development of breast cancer cells. OIP5-AS1 knockdown depleted tumor growth in vivo. Conclusions: OIP5-AS1 knockdown suppressed the progression of breast cancer by inducing GLO1 expression via competitively binding to miR-216a-5p, suggesting that OIP5-AS1 was a hopeful biomarker for the therapy of breast cancer. (c) 2020 Elsevier Inc. All rights reserved.
引用
收藏
页码:501 / 510
页数:10
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