Quantitative response of nitrifying and denitrifying communities to environmental variables in a full-scale membrane bioreactor

被引：30

作者：

Gomez-Silvan, C. ^{[1
]}

Vilchez-Vargas, R. ^{[2
]}

Arevalo, J. ^{[3
]}

Gomez, M. A. ^{[3
]}

Gonzalez-Lopez, J. ^{[1
]}

Pieper, D. H. ^{[2
]}

Rodelas, B. ^{[1
]}

机构：

[1] Univ Granada, Fac Farm, Dept Microbiol, Granada, Spain

[2] Helmholtz Ctr Infect Res, Microbial Interact & Proc Res Grp, Braunschweig, Germany

[3] Univ Granada, Dept Ingn Civil, Granada, Spain

来源：

BIORESOURCE TECHNOLOGY | 2014年 / 169卷

关键词：

Urban wastewater treatment; Reverse-transcription qPCR; AOB; NOB; Multivariate analysis; AMMONIA-OXIDIZING BACTERIA; INDUSTRIAL WASTE-WATER; ACTIVATED-SLUDGE; NITROUS-OXIDE; OPERATIONAL PARAMETERS; MICROBIAL COMMUNITIES; TEMPERATURE; MBR; QUANTIFICATION; DIVERSITY;

D O I：

10.1016/j.biortech.2014.06.089

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The abundance and transcription levels of specific gene markers of total bacteria, ammonia-oxidizing Betaproteobacteria, nitrite-oxidizing bacteria (Nitrospira-like) and denitrifiers (N2O-reducers) were analyzed using quantitative PCR (qPCR) and reverse-transcription qPCR during 9 months in a full-scale membrane bioreactor treating urban wastewater. A stable community of N-removal key players was developed; however, the abundance of active populations experienced sharper shifts, demonstrating their fast adaptation to changing conditions. Despite constituting a small percentage of the total bacterial community, the larger abundances of active populations of nitrifiers explained the high N-removal accomplished by the MBR. Multivariate analyses revealed that temperature, accumulation of volatile suspended solids in the sludge, BOD5, NH4+ concentration and C/N ratio of the wastewater contributed significantly (23-38%) to explain changes in the abundance of nitrifiers and denitrifiers. However, each targeted group showed different responses to shifts in these parameters, evidencing the complexity of the balance among them for successful biological N-removal. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：126 / 133

页数：8

共 35 条

[1] Evidence that ammonia-oxidizing archaea are more abundant than ammonia-oxidizing bacteria in semiarid soils of northern Arizona, USA [J].