Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) in vitro and in planta using Lba4404 strain harboring binary vector pBi2E with dsRNA-suppressor of proline dehydrogenase gene

被引：5

作者：

Tishchenko, O. M. ^{[1
]}

Komisarenko, A. G. ^{[1
]}

Mykhalska, S. I. ^{[1
]}

Sergeeva, L. E. ^{[1
]}

Adamenko, N. I. ^{[1
]}

Morgun, B. V. ^{[1
,2
]}

Kochetov, A. V. ^{[3
]}

机构：

[1] Natl Acad Sci Ukraine, Inst Plant Physiol & Genet, UA-03022 Kiev, Ukraine

[2] Natl Acad Sci Ukraine, Inst Cell Biol & Genet Engn, UA-03680 Kiev, Ukraine

[3] Russian Acad Sci, Inst Cytol & Genet, Novosibirsk 630090, Russia

来源：

CYTOLOGY AND GENETICS | 2014年 / 48卷 / 04期

关键词：

sunflower (Helianthus annuus L.); Agrobacterium-mediated transformation in vitro and in planta; dsRNA-suppressor of proline dehydrogenase; STABLE TRANSFORMATION; STRESS; ARABIDOPSIS; EXPRESSION; TOLERANCE; SALT; METABOLISM; MECHANISMS; ENZYME; PLAY;

D O I：

10.3103/S0095452714040094

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To estimate the efficiency of proline dehydrogenase gene suppression aimed at increasing sunflower (Helianthus annuus L.) tolerance level to water deficiency and salinity, we employed the LBA4404 strain harboring pBi2E with double-stranded RNA-suppressor, produced based on the ProDH1 gene of Arabidopsis. Techniques for Agrobacterium-mediated transformation in vitro and in planta during fertilization of sunflower have been proposed. The genotype-dependent integration of T-DNA in the sunflower genome was demonstrated. PCR-analysis showed that ProDH1 is present in the genomes of inbred lines transformed in planta, as well as in T1- and T2-generations. Significantly, increased levels of accumulation of free L-proline during early stages of in vitro cultivation under normal conditions were demonstrated in transgenic regenerants. The application of lethal doses of stressors (0.4 M mannitol and 2.0% sea salts) caused increase of L-proline level in transgenic regenerants and its decline during the recovery period. This data indicates the effectiveness of suppression of the sunflower ProDH1 gene for increased osmotolerance.

引用

页码：218 / 226

页数：9

共 1 条

[1] Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) in vitro and in planta using Lba4404 strain harboring binary vector pBi2E with dsRNA-suppressor of proline dehydrogenase gene
O. M. Tishchenko
A. G. Komisarenko
S. I. Mykhalska
L. E. Sergeeva
N. I. Adamenko
B. V. Morgun
A. V. Kochetov
Cytology and Genetics, 2014, 48 : 218 - 226

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