Liquid-liquid extraction of xylitol dehydrogenase from Candida guilliermondii homogenate by reversed micelles

被引：11
作者：
Cortez, EV
Pessoa, A
Felipe, MDD
Roberto, IC
Vitolo, M
机构：
[1] Univ Sao Paulo, Biochem & Pharmaceut Technol Dept, FCF, BR-05315970 Sao Paulo, Brazil
[2] FAENQUIL, Dept Biotechnol, BR-12600000 Lorena, SP, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 807卷 / 01期
关键词：
Candida guilliermondii; xylitol dehydrogenase;
D O I：
10.1016/j.jchromb.2004.03.034
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The intracellular enzyme xylitol dehydrogenase (XD, EC 1.1.1.9) from Candida guilliermondii, grown in sugarcane bagasse hydrolysate, was separated by reversed micelles of BDBAC [N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride] cationic surfactant. An experimental design was employed to evaluate the influence of the following factors on the enzyme separation: temperature, co-solvent concentration and surfactant concentration. The results showed that just the temperature did not show significant effect on XD recovery. A model was used to represent the activity recovery and fit the experimental data. Under optimized conditions, the recovery of total activity was about 121%, and the purity increased 2.3-fold. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：55 / 60
页数：6
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