Expansion of bone marrow IFN-α-producing dendritic cells in New Zealand Black (NZB) mice:: High level expression of TLR9 and secretion of IFN-α in NZB bone marrow
被引:35
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作者:
Lian, ZX
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机构:Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
Lian, ZX
Kikuchi, K
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机构:Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
Kikuchi, K
Yang, GX
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机构:Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
Yang, GX
Ansari, AA
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机构:Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
Ansari, AA
Ikehara, S
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机构:Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
Ikehara, S
Gershwin, ME
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机构:Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
Gershwin, ME
机构:
[1] Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Davis, CA 95616 USA
[2] Emory Univ, Sch Med, Dept Pathol, Atlanta, GA 30322 USA
[3] Kansai Med Univ, Dept Pathol 1, Moriguchi, Osaka, Japan
来源:
JOURNAL OF IMMUNOLOGY
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2004年
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173卷
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08期
关键词:
D O I:
10.4049/jimmunol.173.8.5283
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
Patients with systemic lupus erythematosus have elevated IFN-alpha production. Furthermore, sera IFN-alpha levels correlate with disease activity. We have focused our attention on whether this phenotype is also seen in the New Zealand Black (NZB) mice and simultaneously addressed the underlying mechanisms. Specifically, we analyzed: 1) levels of sera IFN-alpha after type A CpG ODN 2216 injection in autoimmunity-prone NZB and control mice, and 2) levels of IFN-alpha synthesized by IFN-alpha-producing dendritic cells (IPDCs) using highly enriched populations of CD11c(+)B220(+) IPDCs derived from NZB and control mice; IPDCs are divided into two subpopulations (CD4(+)CD11c(+)B220(+) and CD4(-)CD11c(+)B220(+)). Our data demonstrate that NZB mice produced higher levels of sera IFN-alpha after type A CpG ODN 2216 injection when compared with control mice (p < 0.01). In addition, the cell numbers, frequency, and TLR9 mRNA levels of CD4(+) and CD4(-) IPDC were markedly increased in the bone marrow (BM) of NZB mice. Upon in vitro stimulation with TLR9 ligand-CpG ODN 2216, higher levels of IFN-alpha were synthesized by IPDCs from the BM of NZB. The major contributor of IFN-alpha was the CD4(-)CD11c(+)B220(+) IPDC subpopulation. Furthermore, NZB BM IPDCs manifest impaired expression of homing chemokine CCR7 and CD62L, and IL-12 production. These data on the functional characteristics of the IPDC lineages explain in part the mechanism of hyper-IFN-alpha production and help clarify the mechanism for the expansion of NZB BM IPDCs.
机构:
Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Yan, Sheng
Yim, Lok Yan
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Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Yim, Lok Yan
Tam, Rachel Chun Yee
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机构:
Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Tam, Rachel Chun Yee
Chan, Albert
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Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Chan, Albert
Lu, Liwei
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Univ Hong Kong, Li Ka Shing Fac Med, Dept Pathol, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Lu, Liwei
Lau, Chak Sing
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Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
Lau, Chak Sing
Chan, Vera Sau-Fong
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Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Li Ka Shing Fac Med, Dept Med, Hong Kong, Hong Kong, Peoples R China
机构:
Natl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico
Montesinos, Juan J.
Lopez-Garcia, Lucero
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机构:
Natl Autonomous Univ Mexico UNAM, Immunol & Stem Cells Lab, FES Zaragoza, Mexico City 09230, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico
Lopez-Garcia, Lucero
Cortes-Morales, Victor A.
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机构:
Natl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico
Cortes-Morales, Victor A.
Arriaga-Pizano, Lourdes
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机构:
Natl Med Ctr IMSS, Specialty Hosp, Med Res Unit Immunochem, Mexico City 06720, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico
Arriaga-Pizano, Lourdes
Valle-Rios, Ricardo
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机构:
Univ Nacl Autonoma Mexico, Res Div, Peripheral Unit Res Canc & Immunol, Fac Med, Mexico City 04360, DF, Mexico
Hosp Infantil Mexico Dr Federico Gomez, Immunol & Prote Res Lab, Mexico City 06720, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico
Valle-Rios, Ricardo
Fajardo-Orduna, Guadalupe R.
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Natl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico
Fajardo-Orduna, Guadalupe R.
Castro-Manrreza, Marta E.
论文数: 0引用数: 0
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机构:
Natl Autonomous Univ Mexico UNAM, Immunol & Stem Cells Lab, FES Zaragoza, Mexico City 09230, DF, MexicoNatl Med Ctr IMSS, Oncol Hosp, Oncol Res Unit, Mesenchymal Stem Cells Lab, Mexico City 06720, DF, Mexico