Simultaneous Determination of Free Fatty Acids and Esterified Fatty Acids in Rice Oil by Gas Chromatography

被引：12

作者：

Ichihara, Ken'ichi ^{[1
,2
]}

Kohsaka, Chihiro ^{[3
]}

Yamamoto, Yoshihiro ^{[3
]}

Masumura, Takehiro ^{[2
]}

机构：

[1] Kyoto Municipal Inst Ind Technol & Culture, Kyoto Integrated Sci & Technol, Bioanal Ctr, 134 Cyudoji Minamicho, Kyoto 6008813, Japan

[2] Kyoto Prefectural Univ, Grad Sch Life & Environm Sci, Kyoto 6068522, Japan

[3] Kyoto Municipal Inst Ind Technol & Culture, 91 Cyudoji Awatacho, Kyoto 6008815, Japan

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 2021年 / 98卷 / 02期

关键词：

Fatty acid dimethylamide; Free fatty acid; Gas chromatography; Nonesterified fatty acid; Rice oil; METHYL-ESTERS;

D O I：

10.1002/aocs.12442

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Free fatty acids (FFA) in crude rice oil were selectively and stoichiometrically derivatized to fatty acid N,N-dimethylamides (FADMA) by catalytic condensation at 45 degrees C, and then esterified fatty acids (eFA) were directly converted to fatty acid methyl esters (FAME) at 37 degrees C. The mixture of FADMA and FAME formed in a single test tube was injected into the capillary column of a gas chromatograph (GC). No mutual contamination occurred between FFA and eFA, and reliability of the method was confirmed by comparison between GC data obtained by this method and by a conventional isolation method. The advantages of the present method are that no FFA isolation procedures are required, the reactions proceed under mild temperature conditions, and FFA and eFA can be analyzed simultaneously by GC.

引用

页码：149 / 155

页数：7

共 10 条

[1] Losses of DHA from high temperatures of columns during GLC of methyl esters of long-chain omega-3 fatty acids [J].