Affinity Tags for Protein Purification

被引:40
作者
Mishra, Vibhor [1 ,2 ]
机构
[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA
[2] Indiana Univ, Howard Hughes Med Inst, Bloomington, IN 47405 USA
关键词
Proteins; affinity tag; purification; chromatography; protease; solubility; MALTOSE-BINDING PROTEIN; ESCHERICHIA-COLI; FUSION PROTEINS; STREP-TAG; THROMBIN SPECIFICITY; EXPRESSION; TECHNOLOGY; CLEAVAGE; SUMO; PEPTIDE;
D O I
10.2174/1389203721666200606220109
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affmity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affmity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affmity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.
引用
收藏
页码:821 / 830
页数:10
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