Electrochemical camera chip for simultaneous imaging of multiple metabolites in biofilms

被引：95

作者：

Bellin, Daniel L. ^{[1
]}

Sakhtah, Hassan ^{[2
]}

Zhang, Yihan ^{[1
]}

Price-Whelan, Alexa ^{[2
]}

Dietrich, Lars E. P. ^{[2
]}

Shepard, Kenneth L. ^{[1
]}

机构：

[1] Columbia Univ, Dept Elect Engn, 1300 SW Mudd Bldg,500 West 120th St, New York, NY 10027 USA

[2] Columbia Univ, Fairchild Ctr, Dept Biol Sci, 1212 Amsterdam Ave, New York, NY 10027 USA

来源：

NATURE COMMUNICATIONS | 2016年 / 7卷

基金：

美国国家科学基金会;

关键词：

PSEUDOMONAS-AERUGINOSA PAO1; MASS-SPECTROMETRY; PHENAZINE ANTIBIOTICS; AMPEROMETRIC SENSOR; WORKING ELECTRODES; PYOCYANIN; BIOSYNTHESIS; EXPRESSION; MICROSCOPY; OXYGEN;

D O I：

10.1038/ncomms10535

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Monitoring spatial distribution of metabolites in multicellular structures can enhance understanding of the biochemical processes and regulation involved in cellular community development. Here we report on an electrochemical camera chip capable of simultaneous spatial imaging of multiple redox-active phenazine metabolites produced by Pseudomonas aeruginosa PA14 colony biofilms. The chip features an 8mm x 8mm array of 1,824 electrodes multiplexed to 38 parallel output channels. Using this chip, we demonstrate potential-sweep-based electrochemical imaging of whole-biofilms at measurement rates in excess of 0.2 s per electrode. Analysis of mutants with various capacities for phenazine production reveals distribution of phenazine-1-carboxylic acid (PCA) throughout the colony, with 5-methylphenazine-1-carboxylic acid (5-MCA) and pyocyanin (PYO) localized to the colony edge. Anaerobic growth on nitrate confirms the O-2-dependence of PYO production and indicates an effect of O-2 availability on 5-MCA synthesis. This integrated-circuit-based technique promises wide applicability in detecting redox-active species from diverse biological samples.

引用

页数：10

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