Functional expression and analysis of the pancreatic transcription factor PDX-1 in yeast

被引：5

作者：

Ozcan, S ^{[1
]}

Mosley, AL ^{[1
]}

Aryal, BK ^{[1
]}

机构：

[1] Univ Kentucky, Albert B Chandler Med Ctr, Dept Mol & Cellular Biochem, Lexington, KY 40536 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 295卷 / 03期

关键词：

Pdx-1; transcription factor; yeast; pancreas; activator; gene expression;

D O I：

10.1016/S0006-291X(02)00747-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pancreas-specific transcription factor Pdx-1 is important for pancreas development and beta-cell specific gene expression in insulin-producing cells. We have expressed the mouse PDX-1 gene in the yeast Saccharomyces cerevisiae and characterized its functional domains. Pdx-1 functions as a strong activator in yeast and stimulates gene expression by more than 80-fold. The transcriptional activation domain of Pdx-1 is located within the first 144 amino-terminal amino acids. Pdx-1 is also able to bind and activate transcription from the A3 element of the human insulin gene promoter in yeast. Analysis of the effects of two-point mutations (Q59L and R197H) in the PDX-1 gene found in type 11 diabetes patients showed that both point mutations interfere with the ability of Pdx-1 to bind to DNA and to activate transcription in yeast. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：724 / 729

页数：6

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