Differences among the cell wall galactomannans from Aspergillus wentii and Chaetosartorya chrysella and that of Aspergillus fumigatus

The alkali extractable and water-soluble cell wall polysaccharides F1SS from Aspergillus wentii and Chaetosartorya chrysella have been studied by methylation analysis, 1D- and 2D-NMR, and MALDI-TOF analysis. Their structures are almost identical, corresponding to the following repeating unit: [-->3)-beta-D-Galf-(1-->5)-beta-D-Galf-(1-->](n)-->mannan core. The structure of this galactofuranose side chain differs from that found in the pathogenic fungus Aspergillus fumigatus, in other Aspergillii and members of Trichocomaceae: [-->5)-beta-D-Galf-(1-->](n)-->mannan core. The mannan cores have also been investigated, and are constituted by a (1-->6)-alpha-mannan backbone, substituted at positions 2 by chains from 1 to 7 residues of (1-->2) linked alpha-mannopyranoses.