Frequency and characterization of antigen-specific IL-4-and IL-13-producing basophils and T cells in peripheral blood of healthy and asthmatic subjects

被引:89
作者
Devouassoux, G
Foster, B
Scott, LM
Metcalfe, DD
Prussin, C
机构
[1] NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA
[2] NIH, Bethesda, MD 20892 USA
关键词
basophils; T cell; cytokines; flow cytometry; human; allergy; asthma;
D O I
10.1016/S0091-6749(99)70292-7
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Both basophils and T cells are known to secrete IL-4 and IL-13 after activation with either nonspecific stimuli or specific antigen, but the relative contribution of these 2 cell types to overall cytokine production is unclear. Objectives: To further characterize basophil cytokine production and compare it with that of T cells, we examined the frequency of IL-4- and IL-13-producing basophils and T cells in human PBMCs by means of flow cytometry after activation in allergic asthmatic and normal subjects. Methods: PBMCs obtained from whole blood after Percoll gradient were activated with specific antigen or ionomycin and fixed. PBMCs were made permeable; stained with antibodies to IgE, CD3, and either IL-4 or IL-13; and analyzed by means of flow cytometry, Results: Preformed cytokines were not detected in unactivated basophils, After ionomycin activation, 60% to 90% of basophils from both control and allergic asthmatic subjects expressed IL-4 and IL-13, Specific antigen induced cytokine expression by 10% to 20% of basophils from the asthmatic group only. After specific antigen activation, basophils accounted for 4 times more IL-4-producing cells than did T cells, IL-4 and IL-13 production at 2 hours was exclusively from basophils, After allergen activation, CD40 ligand was upregulated on a subset of peripheral blood basophils, Conclusions: These data demonstrate that basophils are the predominant peripheral blood cells that express IL-4 and IL-13 in the first 6 hours after antigen activation and strengthen the putative role of basophils both in IgE production and in the generation of allergic inflammation.
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页码:811 / 819
页数:9
相关论文
共 46 条
[21]   CD40 AND IGE - SYNERGISM BETWEEN ANTI-CD40 MONOCLONAL-ANTIBODY AND INTERLEUKIN-4 IN THE INDUCTION OF IGE SYNTHESIS BY HIGHLY PURIFIED HUMAN B-CELLS [J].
JABARA, HH ;
FU, SM ;
GEHA, RS ;
VERCELLI, D .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (06) :1861-1864
[22]   DETECTION OF INTRACELLULAR CYTOKINES BY FLOW-CYTOMETRY [J].
JUNG, T ;
SCHAUER, U ;
HEUSSER, C ;
NEUMANN, C ;
RIEGER, C .
JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 159 (1-2) :197-207
[23]   Restoration of cytomegalovirus-specific CD4+ T-lymphocyte responses after ganciclovir and highly active antiretroviral therapy in individuals infected with HIV-1 [J].
Komanduri, KV ;
Viswanathan, MN ;
Wieder, ED ;
Schmidt, DK ;
Bredt, BM ;
Jacobson, MA ;
McCune, JM .
NATURE MEDICINE, 1998, 4 (08) :953-956
[24]   Unstimulated basophils in atopic and nonatopic subjects express intracellular interleukin-4: detection by flow cytometry [J].
Kon, OM ;
Sihra, BS ;
Till, SJ ;
Corrigan, CJ ;
Kay, AB ;
Grant, JA .
ALLERGY, 1998, 53 (09) :891-896
[25]  
Li HM, 1996, J IMMUNOL, V156, P4833
[26]  
Maurer D, 1996, J IMMUNOL, V157, P607
[27]   EXPRESSION OF FUNCTIONAL HIGH-AFFINITY IMMUNOGLOBULIN-E RECEPTORS (FC-EPSILON-RI) ON MONOCYTES OF ATOPIC INDIVIDUALS [J].
MAURER, D ;
FIEBIGER, E ;
REININGER, B ;
WOLFFWINISKI, B ;
JOUVIN, MH ;
KILGUS, O ;
KINET, JP ;
STINGL, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (02) :745-750
[28]   IMMUNOLOCALIZATION OF INTRACELLULAR INTERLEUKIN-4 IN NORMAL HUMAN PERIPHERAL-BLOOD BASOPHILS [J].
MUELLER, R ;
HEUSSER, CH ;
RIHS, S ;
BRUNNER, T ;
BULLOCK, GR ;
DAHINDEN, CA .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1994, 24 (12) :2935-2940
[29]   Counting antigen-specific CD8 T cells: A reevaluation of bystander activation during viral infection [J].
Murali-Krishna, K ;
Altman, JD ;
Suresh, M ;
Sourdive, DJD ;
Zajac, AJ ;
Miller, JD ;
Slansky, J ;
Ahmed, R .
IMMUNITY, 1998, 8 (02) :177-187
[30]  
NONAKA M, 1995, J IMMUNOL, V155, P3234